研究・教育歴
| 2001年 横浜市大大学院総合理学研究科 博士研究員（PI: 白川昌宏 教授）
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| 2002年 生物分子工学研究所 構造解析部 研究員（PI: 楯真一、森川耿右 博士）
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| 2006年 九州大学生体防御医学研究所 博士研究員（PI: 神田大輔 教授）
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| 2008年 奈良先端科学技術大学院大学 生体高分子構造学講座 助教（PI: 児嶋長次郎 准教授）
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| 2013年 奈良先端科学技術大学院大学 植物分子遺伝学研究室 助教（PI: 島本功 教授）
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| 2014年 京都大学 工学研究科 分子工学専攻 特定研究員（PI：白川昌宏 教授）
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専門分野
分子生物学、構造生物学、核磁気共鳴、X線結晶構造解析
主な研究テーマ
- 専門はNMR、X線結晶構造解析を主とした構造生物化学です。多様な疾病の診断や治療法の開発を目指して、原因となっている蛋白質複合体の機能と構造を解析しています。同時に医療や創薬の場（マイクロドーズ試験等）で実際に使えるような、パラ水素、ダイヤモンドNV中心やDNPを用いた超偏極NMR/MRI測定技術の開発も行っています。また、食糧環境問題の克服に向け、植物の花成ホルモン（フロリゲン）の機能構造解析や花成制御技術の開発も進めています。
主な論文・著書
- Fujita H, Tokunaga A, Shimizu S, Whiting AL, Aguilar-Alonso F, Takagi K, Walinda E, Sasaki Y, Shimokawa T, Mizushima T, Ohki I, Ariyoshi M, Tochio H, Bernal F, Shirakawa M, Iwai K., Cooperative Domain Formation by Homologous Motifs in HOIL-1L and SHARPIN Plays A Crucial Role in LUBAC Stabilization. Cell Rep. 23, 1192-1204 (2018) [pubmed]
- Kaneko-Suzuki M, Kurihara-Ishikawa R, Okushita-Terakawa C, Kojima C, Nagano-Fujiwara M, Ohki I, Tsuji H, Shimamoto K, Taoka KI., TFL1-Like Proteins in Rice Antagonize Rice FT-Like Protein in Inflorescence Development by Competition for Complex Formation with 14-3-3 and FD. Plant Cell Physiol. 59, 458-468 (2018) [pubmed]
- Kosami K*, Ohki I*#, Nagano M, Furuita K, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C#. (*co-first authors, #co-corresponding authors), The crystal structure of the plant small GTPase OsRac1 reveals its mode of binding to NADPH oxidase. J Biol Chem. 289, 28569-78 (2014) [pubmed]
- Kosami K, Ohki I#, Hayashi K, Tabata R, Usugi S, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C#. (#co-corresponding authors), Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1. Acta Crystallogr F Struct Biol Commun. 70, 113-5 (2013) [pubmed]
- Hattori Y, Furuita K, Ohki I#, Ikegami T, Fukada H, Shirakawa M, Fujiwara T, Kojima C#. (#co-corresponding authors), Utilization of lysine 13C-methylation NMR for protein-protein interaction studies. J Biomol NMR. 55, 19-31 (2012) [pubmed]
- Taoka K, Ohki I, Tsuji H, Kojima C, Shimamoto K., Structure and function of florigen and the receptor complex. Trends Plant Sci. 18, 287-94 (2013) [pubmed]
- Nakano T, Ohki I, Yokota A, Ashida H., MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila. PLoS One. 8, e67385 (2013) [pubmed]
- Tsuji H, Nakashima C, Taoka K, Ohki I, Kojima C, Inada N, Shimamoto K., In vivo imaging of Florigen Activation Complex (FAC) comprising Hd3a, 14-3-3 and OsFD1 in rice Genes & Genetic Systems 87, 392-392 (2012)
- 田岡健一郎, 大木出, 辻寛之, 児嶋長次郎, 島本功, 花成ホルモンフロリゲンとその受容体の構造解析からみえてきたフロリゲン機能の分子基盤 化学と生物 50, 654-659 (2012)
- Taoka K*, Ohki I*, Tsuji H*, Furuita K, Hayashi K, Yanase T, Yamaguchi M, Nakashima C, Purwestri YA, Tamaki S, Ogaki Y, Shimada C, Nakagawa A, Kojima C, Shimamoto K. (*co-first authors), 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen. Nature. 476, 332-5 (2011) [pubmed]
- Ohki I, Amida H, Yamada R, Sugihara M, Ishigaki T, Tate S., Surface plasmon resonance study on functional significance of clustered organization of lectin-like oxidized LDL receptor (LOX-1). Biochim Biophys Acta. 1814, 345-54. (2011) [pubmed]
- 田岡健一郎、大木出、辻寛之、児嶋長次郎、島本功, 14-3-3タンパク質はフロリゲンの細胞内における受容体としてはたらく ライフサイエンス新着論文レビュー (2011)
- Ohki I, Kojima C, Structure of florigen activation complex SPring-8 research frontiers 2011 30-31 (2011)
- Ohki I, Furuita K, Hayashi K, Taoka K, Tsuji H, Nakagawa A, Shimamoto K, Kojima C, Structural basis for floral induction by rice florigen Hd3a Acta Cryst A 67, C351 (2011)
- Nakamura S, Kuroki K, Ohki I, Sasaki K, Kajikawa M, Maruyama T, Ito M, Kameda Y, Ikura M, Yamamoto K, Matsumoto N, Maenaka K., Molecular basis for E-cadherin recognition by killer cell lectin-like receptor G1 (KLRG1). J Biol Chem. 284, 27327-35 (2009) [pubmed]
- Inomata K, Ohki I, Tochio H, Fujiwara K, Hiroaki H, Shirakawa M., Kinetic and thermodynamic evidence for flipping of a methyl-CpG binding domain on methylated DNA. Biochemistry. 47, 3266-71 (2008) [pubmed]
- Ishigaki T, Ohki I, Utsunomiya-Tate N, Tate SI., Chimeric structural stabilities in the coiled-coil structure of the NECK domain in human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1). J Biochem. 141, 855-66 (2007) [pubmed]
- Ohki I, Ishigaki T, Oyama T, Matsunaga S, Xie Q, Ohnishi-Kameyama M, Murata T, Tsuchiya D, Machida S, Morikawa K, Tate S., Crystal structure of human lectin-like, oxidized low-density lipoprotein receptor 1 ligand binding domain and its ligand recognition mode to OxLDL. Structure. 13, 905-17 (2005) [pubmed]
- Kasai N, Tsunaka Y, Ohki I, Hirose S, Morikawa K, Tate S., Solution structure of the HMG-box domain in the SSRP1 subunit of FACT. J Biomol NMR. 32, 83-8 (2005) [pubmed]
- Ishigaki T, Ohki I, Oyama T, Machida S, Morikawa K, Tate S., Purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1). Acta Crystallogr Sect F Struct Biol Cryst Commun. 61, 524-7. (2005) [pubmed]
- Fujiwara K, Tenno T, Sugasawa K, Jee JG, Ohki I, Kojima C, Tochio H, Hiroaki H, Hanaoka F, Shirakawa M., Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B. J Biol Chem. 279, 4760-7 (2003) [pubmed]
- Watanabe S, Ichimura T, Fujita N, Tsuruzoe S, Ohki I, Shirakawa M, Kawasuji M, Nakao M., Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase link transcriptional repression and DNA repair in chromatin. Proc Natl Acad Sci U S A. 100, 12859-64 (2003) [pubmed]
- Kato Z, Jee J, Shikano H, Mishima M, Ohki I, Ohnishi H, Li A, Hashimoto K, Matsukuma E, Omoya K, Yamamoto Y, Yoneda T, Hara T, Kondo N, Shirakawa M., The structure and binding mode of interleukin-18. Nat Struct Biol. 10, 966-71 (2003) [pubmed]
- Shirakawa M, Ohki I., Recognition of methylated DNA. Tanpakushitsu Kakusan Koso. 47, 1268-75. (2002) [pubmed]
- 白川昌宏、大木出, メチル化DNAの認識機構 蛋白質核酸酵素 (2002)
- Ohki I, Shimotake N, Fujita N, Jee J, Ikegami T, Nakao M, Shirakawa M., Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA. Cell. 105, 487-97 (2001) [pubmed]
- Ikegami T, Okada T, Ohki I, Hirayama J, Mizuno T, Shirakawa M., Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli. Biochemistry. 40, 375-86. (2001) [pubmed]
- 下竹敦哉、大木出、白川昌宏, メチル化CpG結合ドメインの立体構造とDNAとの相互作用 細胞工学 (2001)
- Fujita N, Shimotake N, Ohki I, Chiba T, Saya H, Shirakawa M, Nakao M., Mechanism of transcriptional regulation by methyl-CpG binding protein MBD1. Mol Cell Biol. 20, 5107-18. (2000) [pubmed]
- Ohki I, Shimotake N, Fujita N, Nakao M, Shirakawa M, Solution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1. EMBO J. 18, 6653-61 (1999) [pubmed]
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