Academic career
Research field
Molecular biology, Structural biology, NMR
Current research
Selected publications
- Fujita H, Tokunaga A, Shimizu S, Whiting AL, Aguilar-Alonso F, Takagi K, Walinda E, Sasaki Y, Shimokawa T, Mizushima T, Ohki I, Ariyoshi M, Tochio H, Bernal F, Shirakawa M, Iwai K., Cooperative Domain Formation by Homologous Motifs in HOIL-1L and SHARPIN Plays A Crucial Role in LUBAC Stabilization. Cell Rep. 23, 1192-1204 (2018) [pubmed]
- Kaneko-Suzuki M, Kurihara-Ishikawa R, Okushita-Terakawa C, Kojima C, Nagano-Fujiwara M, Ohki I, Tsuji H, Shimamoto K, Taoka KI., TFL1-Like Proteins in Rice Antagonize Rice FT-Like Protein in Inflorescence Development by Competition for Complex Formation with 14-3-3 and FD. Plant Cell Physiol. 59, 458-468 (2018) [pubmed]
- Kosami K*, Ohki I*#, Nagano M, Furuita K, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C#. (*co-first authors, #co-corresponding authors), The crystal structure of the plant small GTPase OsRac1 reveals its mode of binding to NADPH oxidase. J Biol Chem. 289, 28569-78 (2014) [pubmed]
- Kosami K, Ohki I#, Hayashi K, Tabata R, Usugi S, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C#. (#co-corresponding authors), Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1. Acta Crystallogr F Struct Biol Commun. 70, 113-5 (2013) [pubmed]
- Hattori Y, Furuita K, Ohki I#, Ikegami T, Fukada H, Shirakawa M, Fujiwara T, Kojima C#. (#co-corresponding authors), Utilization of lysine 13C-methylation NMR for protein-protein interaction studies. J Biomol NMR. 55, 19-31 (2012) [pubmed]
- Taoka K, Ohki I, Tsuji H, Kojima C, Shimamoto K., Structure and function of florigen and the receptor complex. Trends Plant Sci. 18, 287-94 (2013) [pubmed]
- Nakano T, Ohki I, Yokota A, Ashida H., MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila. PLoS One. 8, e67385 (2013) [pubmed]
- Tsuji H, Nakashima C, Taoka K, Ohki I, Kojima C, Inada N, Shimamoto K., In vivo imaging of Florigen Activation Complex (FAC) comprising Hd3a, 14-3-3 and OsFD1 in rice Genes & Genetic Systems 87, 392-392 (2012)
- 田岡健一郎, 大木出, 辻寛之, 児嶋長次郎, 島本功, 花成ホルモンフロリゲンとその受容体の構造解析からみえてきたフロリゲン機能の分子基盤 化学と生物 50, 654-659 (2012)
- Taoka K*, Ohki I*, Tsuji H*, Furuita K, Hayashi K, Yanase T, Yamaguchi M, Nakashima C, Purwestri YA, Tamaki S, Ogaki Y, Shimada C, Nakagawa A, Kojima C, Shimamoto K. (*co-first authors), 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen. Nature. 476, 332-5 (2011) [pubmed]
- Ohki I, Amida H, Yamada R, Sugihara M, Ishigaki T, Tate S., Surface plasmon resonance study on functional significance of clustered organization of lectin-like oxidized LDL receptor (LOX-1). Biochim Biophys Acta. 1814, 345-54. (2011) [pubmed]
- 田岡健一郎、大木出、辻寛之、児嶋長次郎、島本功, 14-3-3タンパク質はフロリゲンの細胞内における受容体としてはたらく ライフサイエンス新着論文レビュー (2011)
- Ohki I, Kojima C, Structure of florigen activation complex SPring-8 research frontiers 2011 30-31 (2011)
- Ohki I, Furuita K, Hayashi K, Taoka K, Tsuji H, Nakagawa A, Shimamoto K, Kojima C, Structural basis for floral induction by rice florigen Hd3a Acta Cryst A 67, C351 (2011)
- Nakamura S, Kuroki K, Ohki I, Sasaki K, Kajikawa M, Maruyama T, Ito M, Kameda Y, Ikura M, Yamamoto K, Matsumoto N, Maenaka K., Molecular basis for E-cadherin recognition by killer cell lectin-like receptor G1 (KLRG1). J Biol Chem. 284, 27327-35 (2009) [pubmed]
- Inomata K, Ohki I, Tochio H, Fujiwara K, Hiroaki H, Shirakawa M., Kinetic and thermodynamic evidence for flipping of a methyl-CpG binding domain on methylated DNA. Biochemistry. 47, 3266-71 (2008) [pubmed]
- Ishigaki T, Ohki I, Utsunomiya-Tate N, Tate SI., Chimeric structural stabilities in the coiled-coil structure of the NECK domain in human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1). J Biochem. 141, 855-66 (2007) [pubmed]
- Ohki I, Ishigaki T, Oyama T, Matsunaga S, Xie Q, Ohnishi-Kameyama M, Murata T, Tsuchiya D, Machida S, Morikawa K, Tate S., Crystal structure of human lectin-like, oxidized low-density lipoprotein receptor 1 ligand binding domain and its ligand recognition mode to OxLDL. Structure. 13, 905-17 (2005) [pubmed]
- Kasai N, Tsunaka Y, Ohki I, Hirose S, Morikawa K, Tate S., Solution structure of the HMG-box domain in the SSRP1 subunit of FACT. J Biomol NMR. 32, 83-8 (2005) [pubmed]
- Ishigaki T, Ohki I, Oyama T, Machida S, Morikawa K, Tate S., Purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1). Acta Crystallogr Sect F Struct Biol Cryst Commun. 61, 524-7. (2005) [pubmed]
- Fujiwara K, Tenno T, Sugasawa K, Jee JG, Ohki I, Kojima C, Tochio H, Hiroaki H, Hanaoka F, Shirakawa M., Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B. J Biol Chem. 279, 4760-7 (2003) [pubmed]
- Watanabe S, Ichimura T, Fujita N, Tsuruzoe S, Ohki I, Shirakawa M, Kawasuji M, Nakao M., Methylated DNA-binding domain 1 and methylpurine-DNA glycosylase link transcriptional repression and DNA repair in chromatin. Proc Natl Acad Sci U S A. 100, 12859-64 (2003) [pubmed]
- Kato Z, Jee J, Shikano H, Mishima M, Ohki I, Ohnishi H, Li A, Hashimoto K, Matsukuma E, Omoya K, Yamamoto Y, Yoneda T, Hara T, Kondo N, Shirakawa M., The structure and binding mode of interleukin-18. Nat Struct Biol. 10, 966-71 (2003) [pubmed]
- Shirakawa M, Ohki I., Recognition of methylated DNA. Tanpakushitsu Kakusan Koso. 47, 1268-75. (2002) [pubmed]
- 白川昌宏、大木出, メチル化DNAの認識機構 蛋白質核酸酵素 (2002)
- Ohki I, Shimotake N, Fujita N, Jee J, Ikegami T, Nakao M, Shirakawa M., Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA. Cell. 105, 487-97 (2001) [pubmed]
- Ikegami T, Okada T, Ohki I, Hirayama J, Mizuno T, Shirakawa M., Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli. Biochemistry. 40, 375-86. (2001) [pubmed]
- 下竹敦哉、大木出、白川昌宏, メチル化CpG結合ドメインの立体構造とDNAとの相互作用 細胞工学 (2001)
- Fujita N, Shimotake N, Ohki I, Chiba T, Saya H, Shirakawa M, Nakao M., Mechanism of transcriptional regulation by methyl-CpG binding protein MBD1. Mol Cell Biol. 20, 5107-18. (2000) [pubmed]
- Ohki I, Shimotake N, Fujita N, Nakao M, Shirakawa M, Solution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1. EMBO J. 18, 6653-61 (1999) [pubmed]
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