Faculty Information, Institute for Chemical Research, Kyoto University   [ English | Japanese ]


SUGISAKI, Hiroyuki

Division of Biochemistry - Molecular Biology -
Associate Professor
Doctor of Science
URL:
E-mail: sugisaki@molbio.kuicr.kyoto-u.ac.jp
Tel: 0774-38-3301
Fax: 0774-38-3259
Academic career
Research field
    Molecular Biology
Current research
Selected publications
  1. Li S, Kimura M, Takashima T,Hayashi K, Inoue K, Ishiguro R, Sugisaki H, and Maruyama K, Role of Cysteine Residues in 4-Oxalomesaconate Hydratase from Pseudomonas ochraceae NGJ1 Biosci. Biotechnol. Biochem 71, 449-458 (2007)
  2. Maruyama K, Shibayama T, Icikawa A, Sakou Y, Yamada S, Sugisaki, H: Cloning and Characterization of the Genes Encoding Enzymes for the Protocatechuate , Meta-degradation Pathway of Pseudomonas ochraceae NGJ1. Biosci. Biotechnol. Biochem, 68, 1434-1441 (2004).
  3. Sugisaki H, Takanami M, DNA sequence restricted by restriction endonuclease AP from Haemophilus aphirophilus Nature New Biology 246, 138-140 (1973)
  4. Takanami M, Okamoto T, Sugimoto K, Sugisaki H, Studies on bacteriophage fd DNA. I. A cleavage map of the fd genome J. Mol. Biol. 95, 21-31 (1975)
  5. Okamoto T, Sugimoto K, Sugisaki H, Takanami M, Studies on bacteriophage fd DNA. II. Localization of RNA initiation sites on the cleavage map of the fd genome J. Mol. Biol. 95, 33-44 (1975)
  6. Sugimoto K, Okamoto T, Sugisaki H, Takanami M, The nucleotide sequence of an RNA polymerase binding site on bacteriophage fd DNA Nature 253, 410-414 (1975)
  7. Sugimoto K, Sugisaki H, Okamoto T, Takanami M, Studies on bacteriophage fd DNA. III. Nucleotide sequence preceding the RNA start-site on a promoter-containing fragment Nucleic Acids Res. 2, 2091-2100 (1975)
  8. Sugisaki H, A novel method for sequencing non-radioactive nucleic acids Bull. Inst. Chem. Res. Kyoto Univ. 54, 154-169 (1976)
  9. Takanami M, Sugimoto K, Sugisaki H, Okamoto T, Sequence of promoter for coat protein of bacteriophage fd Nature 260, 297-302 (1976)
  10. Sugimoto K, Sugisaki H, Okamoto T, Takanami M, Studies on bacteriophage fd DNA. IV. The sequence of messenger RNA for major coat protein gene J. Mol. Biol. 111, 487-507 (1977)
  11. Okamoto T, Sugimoto K, Sugisaki H, Takanami M, DNA region essential for the function of bacteriophage fd promoter Nucleic Acids Res. 4, 2213-2222 (1977)
  12. Sugisaki H, Sequence of two restriction fragments containing promoters of bacteriophage fd Bull. Inst. Chem. Res. Kyoto Univ. 55, 310-322 (1977)
  13. Sugisaki H, Recognition sequence of a restriction endonuclease from Haemophilus gallinarum Gene 3, 17-28 (1978)
  14. Beck E, Sommer R, Auerswald E A, Kurz Ch, Zink Z, Osterburg G, Schaller H, Sugimoto K, Sugisaki H, Okamoto T, Takanami M, Nucleotide sequence of bacteriophage fd DNA Nucleic Acids Res. 5, 4495-4503 (1978)
  15. Hirota Y, Yasuda S, Yamada M, Nishimura A, Sugimoto K, Sugisaki H, Oka A, Takanami M, Structure and functional properties of the Escherichia coli origin of DNA replication Cold Spring Harbor Symp. Quant. Biol. 43, 129-138 (1978)
  16. Oka A, Nomura N, Sugimoto K, Sugisaki H, Takanami M, Nucleotide sequence at the insertion sites of kanamycin transposon Nature 276, 845-847 (1978)
  17. Takahashi H, Shimizu M, Saito H, Ikeda Y, Sugisaki H, A new site-specific endonuclease from Streptomyces lavendulae Gene 5, 9-18 (1979)
  18. Sugimoto K, Oka A, Sugisaki H, Takanami M, Nishimura A, Yamada M, Hirota Y, Nucleotide sequence of Escherichia coli K-12 replication origin Proc. Natl. Acad. Sci. USA 76, 575-579 (1979)
  19. Oka A, Nomura N, Morita M, Sugisaki H, Sugimoto K, Takanami M, Structure of the regions essential for autonomous replication and colicin E1 immunity Molec. Gen. Genet. 172, 151-159 (1979)
  20. Kuchino Y, Kato M, Sugisaki H, Nishimura S, Nucleotide sequence of starfish initiator tRNA Nucleic Acids Res. 6, 3459-3469 (1979)
  21. Fujinaga K, Sawada Y, Uemizu Y, Yamashita T, Shimojo H, Shiroki K, Sugisaki H, Sugimoto K, Takanami M, Nucleotide sequences, integration and transcription of the adenovirus type 12 transforming gene Cold Spring Harbor Symp. Quant. Biol. 44, 519-532 (1979)
  22. Sugisaki H, Sugimoto K, Takanami M, Shiroki K, Saito I, Shimojo H, Sawada Y, Uemizu Y, Uesugi S, Fujinaga K, Structure and gene organization in the transforming HindIII-G fragment of Ad12 Cell 20, 777-786 (1980)
  23. Yokota T, Sugisaki H, Takanami M, Kajiro Y, The nucleotide sequence of the cloned tufA gene of Escherichia coli Gene 12, 25-31 (1980)
  24. Oka A, Sugisaki H, Takanami M, Nucleotide Sequence of the kanamycin resistance transposon Tn903 J. Mol. Biol. 147, 217-226 (1981)
  25. Murotsu M, Matsubara K, Sugisaki H, Takanami M, Nine unique repeating sequences in a region essential for replication and incompatibility of mini-F plasmid Gene 15, 73-78 (1981)
  26. Sugisaki H, Kanazawa S, New restriction endonucleases from Flavobacterium okeanokoites (FokI) and Micrococcus luteus (MluI) Gene 16, 73-78 (1981)
  27. Kimura T, Sawada Y, Shinagawa M, Shimizu Y, Shiroki K, Shimojo H, Sugisaki H, Takanami M, Uesugi Y, Fujinaga K, Nucleotide sequence of the transforming early region E1b of adenovirus type 12 DNA: Structure and gene organization and comparison with those of adenovirus type 5 DNA Nucleic Acids Res. 9, 6571-6589 (1981)
  28. Fujiyoshi Y, Yamagishi H, Kunisada T, Sugisaki H, Kobayashi T, Uyeda N, Visualization of the DNA thread packing within bacteriophage T4 heads J. Ultrastructure Res. 79, 235-240 (1982)
  29. Sugisaki H, Maekawa Y, Kanazawa S, Takanami M, New restriction endonuclease from Acetobacter aceti and Bacillus aneurinolyticus Nucleic Acids Res. 10, 5747-5752 (1982)
  30. Sugisaki H, Maekawa Y, Kanazawa S, Takanami M, Screening of type II restriction endonuclease. I. Isolation and characterization of enzymes from fifteen bacterial strains Bull. Inst. Chem. Res. Kyoto. Univ. 60, 328-335 (1982)
  31. Kawarabayashi Y, Sugisaki H, Structure of viral DNA in a rat cell line transformed by the cloned EcoRI-C fragment of adenovirus 12 Nucleic Acids Res. 13, 6591-6604 (1985)
  32. Birkenmeyer L, Sugisaki H, Ray D S, The majority of minicircle DNA in Crithidia fasciculata strain CF-C1 is of single class with nearly homogeneous DNA sequence Nucleic Acids Res. 13, 7107-7118 (1985)
  33. Shibano Y, Yamashita A, Nakamura N, Ishizuka T, Sugisaki H, Takanami M, Nucleotide sequence coding for the insecticidal fragment of the Bacillus thuringiensis crystal protein Gene 34, 243-251 (1985)
  34. Kudo S, Sugisaki H, Takanami M, Yamashita Y, Ishino M, Shimizu Y, Iida Y, Fujinaga K, Structure of viral DNA in rat cell line, GY1, transformed by Ad12 HindIII fragment-G Virology 148, 133-145 (1986)
  35. Ray D S, Hines J C, Sugisaki H, Sheline C, kDNA minicircles of the major sequence class of C. fasciculata contain a single region of bent helix widely separated from the two origins of replication Nucleic Acids Res. 14, 7953-7965 (1986)
  36. Sugisaki H, Ray D S, DNA sequence of Crithidia fasciculata kinetoplast minicircles Mol. Biochem. Parasitol. 23, 253-263 (1987)
  37. Birkenmeyer L, Sugisaki H, Ray D S, Structural characterization of site-specific discontinuities associated with replication origins of minicircle DNA from Crithidia fasciculate J. Biol. Chem. 262, 2384-2392 (1987)
  38. Ray D S, Birkenmeyer L, Sugisaki H, Sequence organization and replication intermediates of the kinetoplast DNA minicircles of Crithidia fasciculata UCLA Symp. Molec. Cell Biol. New Series Molecular Strategies of Parasitic Invasion, 99-112 (1987)
  39. Kita K, Kotani H, Sugisaki H, Takanami M, FokI Restriction-Modification System. I. Cloning and Nucleotide Sequences of Genes Coding for Restriction and Modification Enzymes J. Biol. Chem. 264, 5751-5756 (1989)
  40. Sugisaki H, Kita K, Takanami M, FokI Restriction-Modification System. II. Presence of Two Domains in FokI Methylase Responsible for Modification of Different DNA Strands J. Biol. Chem. 264, 5757-5761 (1989)
  41. Sugisaki H, Yamamoto K, Takanami M, The HgaI Restriction-Modification System Contains Two Cytosine Methylase Genes Responsible for Modification of Different DNA Strands J. Biol. Chem. 266, 13952-13957 (1991)
  42. Sugisaki H, and Takanami M, The 5' Terminal Region of the Apocytochrome b Transcript in Crithidia fasciculata is Successively Edited by Two Guide RNAs in the 3' to 5' Direction J. Biol. Chem. 268, 887-891 (1993)
  43. Sugisaki H, Nucleotide Sequence of the Gene of HgaI Restriction Endonuclease Bull. Inst. Chem. Res. Kyoto. Univ. 71, 338-342 (1993)
  44. Maruyama K, Miwa M, Tsujii N, Nagai T, Tomita N, Harada T, Sobajima H, and Sugisaki H, Cloning, sequencing, and expression of the gene encoding 4-hydroxy-4-methyl-2-oxoglutarate aldolase from Pseudomonas ochraceae NGJ1 Bioscience, Biotechnology, and Biochemistry 65, 2701-2709 (2001)

Update: Apr 02,2012