京都大学化学研究所研究者情報   [ English | Japanese ]


今西 未来 (いまにし みき )

生体機能化学研究系 生体機能設計化学
(薬学研究科 協力講座)
講師
薬学博士
URL:
E-mail: imiki@scl.kyoto-u.ac.jp
Tel:
Fax:

研究・教育歴
    2002.3 京都大学大学院薬学研究科博士課程修了
    2002.3 京都大学博士(薬学)
    2002.4-2003.6 University of California, San Francisco (USA) 博士研究員
    2003.7-2007.3 京都大学化学研究所助手
    2007.4-2015.9 同助教
    2015.10- 同講師
専門分野
    タンパク質化学
主な研究テーマ
  1. 核酸結合蛋白質の分子認識様式の解明
  2. 遺伝子発現制御分子の創製 と体内時計への応用
主な論文・著書
  1. Tsuji, S.; Futaki, S.; Imanishi, M., Sequence-Specific Recognition of Methylated DNA by an Engineered Transcription Activator-Like Effector Protein Chem Commun 52, 14238-14241 (2016)  [pubmed]
  2. Oku, A.; Imanishi, M.; Noshiro, D.; Murayama, T.; Takeuchi, T.; Nakase, I.; Futaki, S., Use of Calmodulin EF-Hand Peptides as Ca2+-Switchable Recognition Tags Biopolymers 108, e22937 (2017)  [pubmed]
  3. 今西未来, 細胞概日時計の人為的制御 化学と生物 54, 382-383 (2016)
  4. Miyajima, R.; Tsuda, Y.; Inokuma, T.; Shigenaga, A.; Imanishi, M.; Futaki, S.; Otaka, A., Preparation of Peptide Thioesters from Naturally Occurring Sequences Using Reaction Sequence Consisting of Regioselective S-Cyanylation and Hydrazinolysis. Biopolymers 106, 531-546 (2016)  [pubmed]
  5. Azuma, Y.; Kukenshoner, T.; Ma, G.; Yasunaga, J.; Imanishi, M.; Tanaka, G.; Nakase, I.; Maruno, T.; Kobayashi, Y.; Arndt, K. M.; Matsuoka, M.; Futaki, S., Controlling Leucine-Zipper Partner Recognition in Cells through Modification of a-g Interactions. Chem Commun (Camb) 50, 6364-7 (2014)  [pubmed]
  6. 今西未来, 体内時計の人工制御:リズム治療への挑戦 化学工業 64, 64-69 (2014)
  7. Tsuji, S.; Futaki, S.; Imanishi, M., Creating a TALE Protein with Unbiased 5'-T Binding. Biochem Biophys Res Commun 441, 262-265 (2013)  [pubmed]
  8. Kawaguchi, Y.; Tanaka, G.; Nakase, I.; Imanishi, M.; Chiba, J.; Hatanaka, Y.; Futaki, S., Identification of Cellular Proteins Interacting with Octaarginine (R8) Cell-penetrating Peptide by Photo-crosslinking. Bioorg Med Chem Lett 23, 3738-3740 (2013)  [pubmed]
  9. Noshiro, D.; Sonomura, K,; Yu, H. H.; Imanishi, M.; Asami, K.; Futaki, S., Construction of a Ca(2+)-gated Artificial Channel by Fusing Alamethicin with a Calmodulin-derived Extramembrane Segment. Bioconjug Chem 24, 188-195 (2013)  [pubmed]
  10. Imanishi, M., Design of Artificial DNA Binding Proteins toward Control and Elucidation of Cellular Functions Yakugaku Zasshi 132, 1431-1436 (2012)  [pubmed]
  11. Imanishi M, Yamamoto K, Yamada H, Hirose Y, Okamura H, Futaki S., Construction of a rhythm transfer system that mimics the cellular clock. ACS Chem Biol 7, 1817-1821 (2012)  [pubmed]
  12. Nakase, I.; Okumura, S.; Tanaka, G.; Osaki, K.; Imanishi, M.; Futaki, S., Signal Transduction Using an Zrtificial Receptor System That Undergoes Dimerization upon Addition of a Bivalent Leucine-Zipper Ligand Angew Chem Int Ed Engl 51, 7464-7467 (2012)  [pubmed]
  13. Azuma, Y.; Imai, H.; Yoshimura, T.; Kawabata, T.; Imanishi, M.; Futaki, S., Dipicolylamine as a Unique Structural Switching Element for Helical Peptides. Org Biomol Chem 10, 6062-6068 (2012)  [pubmed]
  14. Imanishi, M.; Matsumura, K.; Tsuji, S.; Nakaya, T.; Negi, S.; Futaki, S.; Sugiura, Y., Zn(II) Binding and DNA Binding Properties of Ligand-Substituted CXHH-Type Zinc Finger Proteins Biochemistry 51, 3342-3348 (2012)  [pubmed]
  15. Kono, H.; Imanishi, M.; Negi, S.; Tatsutani, K.; Sakaeda, Y.; Hashimoto, A.; Nakayama, C.; Futaki, S.; Sugiura, Y., Rational Design of DNA Sequence-Specific Zinc Fingers FEBS Lett 586, 918-923 (2012)  [pubmed]
  16. 今西未来, ゲノムを標的とした人工ジンクフィンガータンパク質の創製 ファルマシア(日本薬学会) 48, 201-205 (2012)
  17. Imanishi, M.; Sugiura, Y., Design of Artificial DNA Binding Proteins toward Control and Elucidation of Cellular Functions Zinc Fingers: Structure, Properties and Applications 141-154 (2012)
  18. Imanishi, M.; Nakamura, A.; Doi, M.; Futaki, S.; Okamura, H., Control of Circadian Phase by an Artificial Zinc Finger Transcription Regulator. Angew Chem Int Ed Engl 50, 9396-9399 (2011)  [pubmed]
  19. Looi, C. Y.; Imanishi, M.; Takaki, S.; Sato, M.; Chiba, N.; Sasahara, Y.; Futaki, S.; Tsuchiya, S.; Kumaki, S., Octa-Arginine Mediated Delivery of Wild-Type Lnk Protein Inhibits TPO-Induced M-MOK Megakaryoblastic Leukemic Cell Growth by Promoting Apoptosis. PLoS One 6, e23640 (2011)  [pubmed]
  20. Negi, S.; Imanishi, M.; Sasaki, M.; Tatsutani, K.; Futaki, S.; Sugiura, Y., An Arginine Residue Instead of a Conserved Leucine Residue in the Recognition Helix of the Finger 3 of Zif268 Stabilizes the Domain Structure and Mediates DNA Binding. Biochemistry 50, 6266-6272 (2011)  [pubmed]
  21. Imanishi M, Negi S, Sugiura Y., Non-FokI-based zinc finger nucleases Methods Mol Biol. 649, 337-349 (2010)  [pubmed]
  22. Imanishi M, Imamura C, Higashi C, Yan W, Negi S, Futaki S, Sugiura Y., Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Sp1 Biochem Biophys Res Commun. 400, 625-630 (2010)  [pubmed]
  23. Imanishi M, Nakaya T, Morisaki T, Noshiro D, Futaki S, Sugiura Y., Metal-stimulated regulation of transcription by an artificial zinc-finger protein Chembiochem 11, 1653-1655 (2010)  [pubmed]
  24. Yu HH, Nakase I, Pujals S, Hirose H, Tanaka G, Katayama S, Imanishi M, Futaki S., Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery Biochim Biophys Acta 1798, 2249-2257 (2010)  [pubmed]
  25. Morisaki T, Imanishi M, Futaki S, Sugiura Y., Artificial transcription factors based on multi-zinc finger motifs Yakugaku Zasshi 130, 45-48 (2010)  [pubmed]
  26. Imanishi M, Nakamura A, Morisaki T, Futaki S., Positive and negative cooperativity of modularly assembled zinc fingers Biochem Biophys Res Commun. 387, 440-443 (2009)  [pubmed]
  27. Azuma Y, Imanishi M, Yoshimura T, Kawabata T, Futaki S, Cobalt(II)-responsive DNA binding of a GCN4-bZIP protein containing cysteine residues functionalized with iminodiacetic acid Angew Chem Int Ed Engl. 48, 6853-6856 (2009).  [pubmed]
  28. Kobayashi S, Nakase I, Kawabata N, Yu HH, Pujals S, Imanishi M, Giralt E, Futaki S, Cytosolic targeting of macromoleculesusing a pH-dependent fusogenic peptide in combination with cationic liposomes Bioconjug Chem. 20, 953-959 (2009)  [pubmed]
  29. Dhanasekaran M, Negi S, Imanishi M, Suzuki M, Sugiura Y., Effects of bulkiness and hydrophobicity of an aliphatic amino acid in the recognition helix of the GAGA zinc finger on the stability of the hydrophobic core and DNA binding affinity. Biochemistry 47, 11717-11724 (2008).  [pubmed]
  30. Morisaki T, Imanishi M, Futaki S, Sugiura Y, Rapid Transcriptional Activity in Vivo and Slow DNA Binding in Vitro by Artificial Multi-Zinc Finger Protein Biochemistry 47, 10171-10177 (2008).  [pubmed]
  31. Negi S, Imanishi M, Matsumoto M, Sugiura Y., New redesigned zinc-finger proteins: design strategy and its application. Chemistry 14, 3236-49 (2008).  [pubmed]
  32. Imanishi, M, Artificial zinc finger proteins: desired DNA sequence-specific function toward gene regulation Drug Design Research Perspectives (Nova Science Publishers, Inc.) , 25-37 (2007).
  33. Yan W, Imanishi M, Futaki S, Sugiura Y, Alpha-Helical Linker of an Artificial 6-Zinc Finger Peptide Contributes to Selective DNA Binding to a Discontinuous Recognition Sequence Biochemistry 46, 8517-8524 (2007)  [pubmed]
  34. Dhanasekaran M, Negi S, Imanishi M, Sugiura Y, DNA-Binding Ability of GAGA Zinc Finger Depends on the Nature of Amino Acids Present in the Beta-Hairpin Biochemistry 46, 7506-7513 (2007).  [pubmed]
  35. Imanishi M, Endres N F, Gennerich A, Vale R D, Auto-Inhibition Regulates the Motility of the C. Elegans Intraflagellar Transport Motor, OSM-3. J. Cell. Biol. 174, 931-937 (2006).
  36. 今西 未来 , 遺伝子を標的とした人工亜鉛フィンガータンパク質の創製 化学工業(化学工業社) 57, 667-671 (2006).
  37. Imanishi M, Yan W, Morisaki T, Sugiura Y , An artificial six-zinc finger peptide with polyarginine linker: Selective binding to the discontinuous DNA sequences Biochemical and Biophysical Research Communications 333, 137-173 (2005).
  38. Hirata T, Nomura W, Imanishi M, Sugiura Y, Effects of linking 15-zinc finger domains on DNA binding specificity and multiple DNA binding modes Bioorg Med Chem Lett 15, 2197-2201 (2005).
  39. Nakatsukasa T, Shiraishi Y, Negi S, Imanishi M, Futaki S, Sugiura Y, Site-specific DNA cleavage by artificial zinc finger-type nuclease with cerium-binding peptide Biochemical and Biophysical Research Communications 330, 247-252 (2005).
  40. Shiraishi Y, Imanishi M, Morisaki T, Sugiura Y, Swapping of the beta-hairpin region between Sp1 and GLI zinc fingers: significant role of the beta-hairpin region in DNA binding properties of C2H2-type zinc finger peptides Biochemistry 44, 2523-2528 (2005).
  41. Negi S, Itazu M, Imanishi M, Nomura A, Sugiura Y, Creation and characteristics of unnatural CysHis3-type zinc finger protein Biochemical and Biophysical Research Communications 325, 421-425 (2004).
  42. Shiraishi Y, Imanishi M, Sugiura Y, Exchange of Histidine Spacing between Sp1 and GLI Zinc Fingers: Distinct Effect of Histidine Spacing-Linker Region on DNA binding Biochemistry 43, 6352-6359 (2004).
  43. Miyagawa N, Sasaki D, Matsuoka M, Imanishi M, Ando T, Sugiura Y, DNA Cleavage Characteristics of Non-Protein Enediyne Antibiotic N1999A2 Biochem. Biophys. Res. Commun. 306, 87-92 (2003).
  44. Imanishi M, Sugiura Y, Artificial DNA-Bending Six-Zinc Finger Peptides with Different Charged Linkers: Distinct Kinetic Properties of DNA Bindings Biochemistry 41, 1328-1334 (2002).
  45. Imanishi M, Hori Y, Nagaoka M, Sugiura Y, Design of Novel Zinc Finger Proteins: Towards Artificial Control of Specific Gene Expression Eur. J. Pharm. Sci. 13, 91-97 (2001).
  46. Nagaoka M, Kaji T, Imanishi M, Hori Y, Nomura W, Sugiura Y, Multiconnection of Identical Zinc Finger: Implication for DNA Binding Affinity and Unit Modulation of the Three Zinc Finger Domain Biochemistry 40, 2932-2941 (2001).
  47. Imanishi M, Hori Y, Nagaoka M, Sugiura Y, DNA-Bending Finger: Artificial Design of 6-Zinc Finger Peptides with Polyglycine Linker and Induction of DNA Bending Biochemistry 39, 4383-4390 (2000).
  48. Kamiuchi T, Abe E, Imanishi M, Kaji T, Nagaoka M, Sugiura Y, Artificial Nine Zinc-Finger Peptide with 30 Base Pair Binding Sites Biochemistry 37, 13827-13834 (1998).
  49. 今西 未来、杉浦 幸雄 , 新規亜鉛フィンガータンパク質の創製 化学 53, 72-73 (1998).


Update: Jul 18,2017