Faculty Information, Institute for Chemical Research, Kyoto University   [ English | Japanese ]


FUTAKI, Shiroh

Division of Biochemistry - Biofunctional Design-Chemistry -
(Laboratory in the Graduate School of Pharmaceutical Sciences, Kyoto University)
Professor
Ph. D.
URL:
E-mail: futaki@scl.kyoto-u.ac.jp
Tel: 0774-38-3210
Fax: 0774-32-3038

Academic career
    1983.4-1987.3 Graduate School of Pharmaceutical Sciences, Kyoto University
    1987.4-1993.5 Research Associate, University of Tokushima
    1989.7 D. Pharm. Sci., Kyoto University
    1993.6-1997.10 Associate Professor, University of Tokushima
    1997.11-2005.3 Associate Professor, Kyoto University, Institute for Chemical Research
    2005.4- Professor, Kyoto University, Institute for Chemical Research
Research field
    Peptide & Protein Engineering, In-Cell Chemistry, Drug Delivery
Current research
  1. Design and creation of bioactive proteins that regulate cell function and genes
  2. Development of cell permeable peptide vectors and elucidation of the internalization mechanisms
  3. Chemistry of the intracellular targeting
  4. Creation of artificial sensor peptides and proteins
Selected publications
  1. Nakase, I.; Osaki, K.; Tanaka, G.; Utani, A.; Futaki, S., Molecular Interplays Involved in the Cellular Uptake of Octaarginine on Cell Surfaces and the Importance of Syndecan-4 Cytoplasmic V Domain for the Activation of Protein Kinase Cα Biochem. Biophys. Res. Commun.446(4), 857-862(2014).
  2. Azuma, Y.; Kukenshoner, T.; Ma, G.; Yasunaga, J.; Imanishi, M.; Tanaka, G.; Nakase, I.; Maruno, T.; Kobayashi, Y.; Arndt, K, M.; Matsuoka, M.; Futaki, S., Controlling Leucine-Zipper Partner Recognition in Cells through Modifications of a-g Interactions Chem. Commun.50(48), 6364-6367(2014).
  3. Kawamoto, S.; Takasu, M.; Miyakawa, T.; Akiyama, R.; Oda, T.; Saito, H.; Futaki, S.; Nagao, H.; Shinoda, W., Free Energy of Cell-Penetrating Peptide through Lipid Bilayer Membrane; Coarse-Grained Model Simulation Progress in Theoretical Chemistry and Physics26, 503-511(2012).
  4. Tsuji, S.; Futaki, S.; Imanishi, M., Creating a TALE Protein with Unbiased 5'-T Binding Biochem. Biophys. Res. Commun.441(1), 262-265(2013).
  5. Mishiro, K.; Furuta, T.; Sasamori, T.; Hayashi, K.; Tokitoh, N.; Futaki, S.; Kawabata, T., A Cyclochiral Conformational Motif Constructed Using a Robust Hydrogen Bonding Network J. Am. Chem. Soc.135(37), 13644-13647 (2013).
  6. Pujals, Sílvia.; Miyamae, H.; Afonin, S.; Murayama, T.; Hirose, H.; Nakase, I.; Taniuchi, K.; Umeda, M.; Sakamoto, K.; Ulrich, A.; Futaki, S., Curvature Engineering; Positive Membrane Curvature Induced by Epsin N-Terminal Peptide Boosts Internalization of Octaarginine ACS Chem. Biol.8(9), 1894-1899 (2013).
  7. Gehin, C.; Montenegro, J.; Bang, E, -K.; Cajaraville, A.; Takayama, S.; Hirose, H.; Futaki, S.; Matile, S.; Riezman, H., Dynamic Amphiphile Libraries to Screen for the “Fragrant” Delivery of siRNA into HeLa Cells and Human Primary Fibroblasts J. Am. Chem. Soc.135(25), 9295-9298 (2013).
  8. Mitsueda, A.; Shimatani, Y.; Ito, M.; Ohgita, T.; Yamada, A.; Hama, S.; Gräslund, A.; Lindberg, S.; Langel, Ü.; Harashima, H.; Nakase, I.; Futaki, S.; Kogure K., Development of a Novel Nanoparticle by Dual Modification with the Pluripotential Cell-Penetrating Peptide PepFect6 for Cellular Uptake, Endosomal Escape and Decondensation of an siRNA Core Complex Biopolymers-Peptide Science100(6), 698-704 (2013).
  9. Katayama, S.; Nakase, I.; Yano, Y.; Murayama, T.; Nakata, Y.; Matsuzaki, K.; Futaki, S., Effects of Pyrenebutyrate on the Translocation of Arginine-rich Cell-penetrating Peptides through Artificial Membranes; Recruiting Peptides to the Membranes, Dissipating liquid-ordered Phases, and Inducing Curvature Biochim. Biophys. Acta1828(4), 1198-1204 (2013).
  10. Kawaguchi, Y.;Tanaka, G.; Nakase, I.; Imanishi, M.; Chiba, J.; Hatanaka, Y.; Futaki, S., Identification of Cellular Proteins Interacting with Octaarginine (R8) Cell-penetrating Peptide by Photo-crosslinking Bioorg. Med. Chem. Lett.23(13), 3738-3740 (2013).
  11. Yamazaki, C, M.; Nakase, I.; Endo, H.; Kishimoto, S.; Mashiyama, Y.; Masuda, R.; Futaki, S.; Koide, K., Collagen-like Cell-penetrating Peptides Angew. Chem. Int. Ed. Engl.52(21), 5497-5500 (2013).
  12. Noshiro, D.; Sonomura, K.; Yu, H, -H.; Imanishi, M.; Asami, K.; Futaki, S., Construction of a Ca2+-gated Artificial Channel by Fusing Alamethicin with a Calmodulin-derived Extramembrane Segment Bioconjug. Chem. 24(2), 188-195 (2013).
  13. Madani, F.; Abdo, R.; Lindberg, S.; Hirose, H.; Futaki, S.; Langel, Ü.; Gräslund, A., Modeling the Endosomal Escape of Cell-Penetrating Peptides Using a Transmembrane pH Gradient Biochim. Biophys. Acta. 1828(4), 1198-1204 (2013).
  14. Takeuchi, T.; Popiel, H. A.; Futaki, S.; Wada, K.; Nagai,Y., Peptide-based therapeutic approaches for treatment of the polyglutamine diseases Curr. Med. Chem.21(23), 2575-2582 (2014).
  15. Futaki, S.; Noshiro, D.; Kiwada, T.; Asami, K., Extramembrane control of ion channel peptide assemblies, using alamethicin as an example Acc. Chem. Res.46(12), 2924-2933 (2013).
  16. Nakase, I.;Tanaka, G.; Futaki, S., Cell-penetrating Peptides (CPPs) as a Vector for the Delivery of siRNAs into Cells Mol. BioSyst.9(5),855-861 (2013).
  17. Futaki, S.; Hirose,H.; Nakase, I., Arginine-rich peptides; methods of translocation through biological membranes Curr. Pharm. Design 19(16), 2863-2868 (2013).
  18. Nakase, I.; Akita, H.; Kogure, K.; Gräslund, A.;.; Ülo Langel.; Harashima, H. Futaki, S., Efficient Intracellular Delivery of Nucleic Acid Pharmaceuticals Using Cell-Penetrating Peptides Acc. Chem. Res. 45(7), 1132-1139 (2012).
  19. Kurod, Y.; Kato-Kogoe, N.; Tasaki, E.; Murata, E.; Ueda, K.; Abe, M.; Miyamoto, K.; Nakase, I.; Futaki, S.; Tohyama, Y.; Hirose, M., Oligopeptides Derived from Autophosphorylation Sites of EGF Receptor Suppress EGF-stimulated Responses in Human Lung Carcinoma A549 cells Eur. J. Pharma.698(1-3),87-94 (2013).
  20. Nakase, I.; Okumura, S.; Katayama, S.; Hirose,H.; Pujals, S.; Yamaguchi, H.; Arakawa, S.; Shimizu, S.; Futaki, S., Transformation of an Antimicrobial Peptide into a Plasma Membrane-permeable, Mitochondria-targeted Peptide via the Substitution of Lysine with Arginine Chem. Commun 48(90), 11097-11099 (2012).
  21. Ueda, Y.; Fan-Yan Wei.; Hide, T.; Michiue, H.; Takayama, K.; Kaitsuka, T.; Nakamura, H.; Makino, K.; Kuratsu, J.; Futaki, S.; Tomizawa, K., Cell-penetrating D-isomer Peptides Consisting of Pas and the p53 C-terminus Induce Autophagic Cell Death of Glioma-initiating Cells Biomaterials 33(35), 9061-9069 (2012).
  22. Imanishi, M.; Yamamoto, K.; Yamada, H.; Hirose, Y.; Okamura, H.; Futaki, S., Construction of a Rhythm Transfer System That Mimics the Cellular Clock ACS Chem. Biol 7(11), 1817-1821(2012).
  23. Noshiro, D.; Asami, K.; Futaki, S., Control of Leakage Activities of Alamethicin Analogs by Metals; Aide Chain-dependent Adverse Gating Response to Zn2 Bioorg. Med. Chem 20(23), 6870-6876 (2012).
  24. Tanaka, G.; Nakase, I.; Fukuda, Y.; Masuda, R.; Oishi, S.; Shimura, K.; Kawaguchi, Y.; Takatani-Nakase, T.; Ülo Langel.; Gräslund, A.;.; Okawa, K.; Matsuoka, M.; Fujii, N.; Hatanaka, Y.; Futaki, S., CXCR4 Stimulates Macropinocytosis; Implications for Cellular Uptake of Arginine-rich Cell-penetrating Peptides and HIV Chem. Biol 19(11), 1437-1446 (2012).
  25. Sakamoto, K.; Aburai, K.; Morishita, T.; Sakai, K.; Sakai, H.; Abe, M.; Nakase, I.; Futaki, S., Bio-inspired Mechanism for the Translocation of Peptide through the Cell-membrane Chem. Lett 41(10), 1078-1080 (2012).
  26. Azuma, Y.; Imai, H.; Yoshimura, T.; Kawabata, T.; Imanishi, M.; Futaki, S., Dipicolylamine as a Unique Structural Switching Element for Helical Peptides Org. Biomol. Chem 10(30), 6062-6068 (2012).
  27. Futaki, S.; Nakase, I.; Okumura, S.; Tanaka, G.; Osaki, K.; Imanishi, M.; Futaki, S., Signal Transduction of Biological Systems through an Artificial Receptor System via Dimerization of a Bivalent Leucine-Zipper Ligand Angew. Chem. Int. Ed. Engl 51(30), 7464-7467 (2012).
  28. Imanishi, M.; Matsumura, K.; Tsuji, S.; Nakaya, T.; Negi, S.; Futaki, S.; Sugiura, Y. , Zn(II)-binding and DNA-binding Properties of Ligand-substituted CXHH-type Zinc Finger Proteins Biochemistry 51(16), 3342-3348 (2012).
  29. Kono, H.; Imanishi, M.; Negi, S.; Tatsutani, K.; Sakaeda, Y.; Hashimoto, A.; Nakayama, C.; Futaki, S.; Sugiura,Y., Rational Design of DNA Sequence-specific Zinc Fingers FEBS Lett 586(6), 918-923 (2012).
  30. Takayama, K.; Hirose,H.; Tanaka, G.; Pujals, Sílvia.; Katayama, S.; Nakase, I.; Futaki, S., The Effect of the Attachment of a Penetration Accelerating Sequence and the Influence of Hydrophobicity on Octaarginine-mediated Intracellular Delivery Mol. Pharm 9(5), 1222-1230 (2012).
  31. Nakase, I.; Konishi, Y.; Ueda, M.; Saji, H.; Futaki, S., Accumulation of Arginine-rich Cell-penetrating Peptides in Tumors and the Potential for Anticancer Drug Delivery in vivo J. Control. Release 159(2), 181-188 (2012).
  32. Hirose,H.; Takeuchi, T.; Osakada, H.; Pujals, Sílvia.; Katayama, S.; Nakase, I.; Kobayashi, S.; Haraguchi, T.; Futaki, S., Transient Focal Membrane Deformation Induced by Arginine-rich Peptides Leads to Their Direct Penetration into Cells Mol. Ther 20(5), 984-993 (2012).
  33. KAWAMOTO, Shuhei.; MIYAKAWA, Takeshi.; TAKASU, Masako.; MORIKAWA, Ryota.; ODA, Tatsuki.; SAITO, Hiroaki.; Futaki, S.; NAGAO, Hidemi., Cell Penetrating Peptide Induces Various Deformations of Lipid Bilayer Membrane; Inverted Micelle, Double Bilayer and Trans-membrane Int. J. Quantum Chem 112(1), 178-183 (2012).
  34. OCHIAI, Tsuyoshi.; NAGATA, Morio.; SHIMOYAMA, Kosuke.; KATO, Tomoya.; ASAOKA, Takahide.; KONDO, Masaharu.; DEWA, Takehisa.; YAMASHITA, Keiji.; KASHIWADA, Ayumi.; FUTAKI, Shiroh.; HASHIMOTO, Hideki.; NANGO, Mamoru., Two-Dimensional Molecular Assembly of Bacteriochlorophyll a Derivatives Using Synthetic Poly(ethylene glycol)-Linked Light-Harvesting Model Polypeptides on a Gold Electrode Modified with Supported Lipid Bilayers ACS Macro Letters 1(1), 28-32 (2011).
  35. IMANISHI, Miki.; NAKAMUEA, Atsushi.; DOI, Masao.; FUTAKI, Shiroh.; OKAMURA, Hitoshi., Control of Circadian Phase by an Artificial Zinc Finger Transcription Regulator. Angew. Chem. Int. Ed. Engl 50(40), 9396-9399 (2011).
  36. LOOI, Chung Yeng.; IMANISHI, Miki.; TAKAKI, Satoshi.; SATO, Miki.; CHIBA, Natsuko,; SASAHARA, Yoji.; FUTAKI, Shiroh.; TSUCHIYA, Shigeru.; KUMAKI, Satoru., Octa-Arginine Mediated Delivery of Wild-Type Lnk Protein Inhibits TPO-Induced M-MOK Megakaryoblastic Leukemic Cell Growth by Promoting Apoptosis PLosOne 6(8), e23640 (2011).
  37. NEGI, Shigeru.; IMANISHI, Miki.; SASAKI, Maeko.; TATSUTANI, Kazuya.; FUTAKI, Shiroh.; SUGIURA, Yukio., An Arginine Residue Instead of a Conserved Leucine Residue in the Recognition Helix of the Finger 3 of Zif268 Stabilizes the Domain Structure and Mediates DNA Binding Biochemistry50(28), 6266-6272 (2011).
  38. SHAHEEN, Sharif M.; AKITA, Hidetaka.; NAKAMURA, Takashi.; TAKAYAMA, Shota.; FUTAKI, Shiroh.; YAMASHITA, Atsushi.; KATOONO, Ryo.; YUI, Nobuhiko.; HARASHIMA, Hideyoshi., KALA-modified Multi-layered Nanoparticles as Gene Carriers for MHC Class-I Mediated Antigen Presentation for a DNA Vaccine Biomaterials 32(26), 6342-6350 (2011).
  39. SAKURAI, Yu.; HATAKEYAMA, Hiroto.; SATO, Yusuke.; AKITA, Hidetaka.; TAKAYAMA, Kentaro.; KOBAYASHI, Sachiko.; FUTAKI, Shiroh.; HARASHIMA, Hideyoshi., Endosomal Escape and the Knockdown Efficiency of Liposomal-siRNA by the Fusogenic Peptide shGALA Biomaterials 32(24), 5733-5742 (2011).
  40. Kawamoto S, Takasu M, Miyakawa T, Morikawa R, Oda T, Futaki S, Nagao H, Inverted Micelle Formation of Cell-penetrating Peptide Studied by Coarse-grained Simulation; Importance of Attractive Force between Cell-penetrating Peptides and Lipid Head Group J. Chem. Phys 34(9), 095103 (2011).
  41. Sonomura K, Kuyama H, Matsuo E, Tsunasawa S, Futaki S, Nishimura O, Selective Isolation of N-blocked Peptide by combining AspN Digestion, Transamination, and Tosylhydrazine Glass Treatment Anal. Biochem 410(2), 214-223 (2011).
  42. Madani F, Lindberg S, Langel U, Futaki S, Gräslund A, Mechanisms of Cellular Uptake of Cell Penetrating Peptides J. Biophys , 414729 (2011).
  43. Katayam S, Hirose H, Takayama K, Nakase I, Futaki S, Acylation of Octaarginine; Implication to the Use of Intracellular Delivery Vectors J. Control. Release , (2010).
  44. KAWAMOTO, Shuhei.; TALASU, Masako,; MIYAKAWA, Takeshi.; MORIKAWA, Ryota.; ODA, Tatsuki.; FUTAKI, Shiroh.; NOGAO, Hidemi., Binding of Tat peptides on DOPC and DOPG lipid bilayer membrane studied by molecular dynamics simulations Molecular Simulation DOI;10.1080/08927022.2010.536546(2010)
  45. Nakase I, Kobayashi S, Futaki S, Endosome-disruptive peptides for improving cytosolic delivery of bioactive macromolecules Biopolymers (Peptide Science)94(6), 763-770 (2010).
  46. Miyamoto R, Akizawa H, Nishikawa T, Uehara T, Azuma Y, Nakase I, Futaki S, Hanaoka H, Iida Y, Endo K, Arano Y, Enhanced Target-Specific Accumulation of Radiolabeled Antibodies by Conjugating Arginine-Rich Peptides as Anchoring Molecules Bioconjug. Chem, (2010).
  47. Imanishi M, Imamura C, Higashi C, Yan W, Negi S, Futaki S, Sugiura Y, Zinc Finger-Zinc Finger Interaction between the Transcription Factors, GATA-1 and Sp1 Biochem. Biophys. Res. Commun. 400(4), 625-630 (2010).
  48. Yukawa H, Noguchi H, Nakase I, Miyamoto Y, Oishi K, Hamajima N, Futaki S, Hayashi S, Transduction of Cell-Penetrating Peptides into Induced Pluripotent Stem Cells Cell Transplant 19(6), 901-909 (2010).
  49. Imanishi M, Nakaya T, Morisaki T, Noshiro D, Futaki S, Sugiura Y, Metal-Stimulated Transcriptional Regulation by an Artificial Zinc Finger Protein ChemBioChem 11(12), 1653-1655 (2010).
  50. Shimane K, Kodama E, Nakase I, Futaki S, Sakurai Y, Sakagami Y, Stefan G. Sarafianos, Matsuoka M, Rev-derived Peptides Inhibit HIV-1 Replication by Antagonism of Rev and a Co-receptor, CXCR4 Int. J. Biochem. Cell Biol. 42(9), 1482-1488 (2010).
  51. Yanaka S, Kudou M, Tanaka Y, Sasaki T, Takemoto S, Sakata A, Hattori Y, Koshi T, Futaki S, Tsumoto K, Nakashima T, Contribution of the Flexible Loop Region to the Function of Staphylococcal Enterotoxin B Protein Eng. Des. Sel. 23(5), 415-421 (2010).
  52. Yu H, Nakase I, Pujals S, Hirose H, Tanaka G, Katayam S, Imanishi M, Futaki S, Expressed Protein Ligation for the Preparation of Fusion Proteins with Cell Penetrating Peptides for Endotoxin Removal and Intracellular Delivery Biochim. Biophys. Acta. 798(12), 2249-2257 (2010).
  53. Akita H, Kogur K, Moriguchi R, Nakamura Y, Higashi T, Nakamura T, Serada S, Fujimoto M, Naka T, Futaki S, Harashima H, Nanoparticles for ex vivo siRNA Delivery to Dendritic Cells for Cancer Vaccines; Programmed Endosomal Escape and Dissociation J. Control. Release 143(3), 311-317 (2010).
  54. Noshiro D, Asami K, Futaki S, Metal-Assisted Channel Stabilization; Disposition of a Single Histidine on the N-terminus of Alamethicin Yielded Channels with Extraordinarily Long Biochem. J. 98(9), 1801-1808 (2010).
  55. Araki D, Takayama K, Inoue M, Watanabe T, KumonvH, Futaki S, Matsui H, Tomizawa K, Cell-Penetrating d-Isomer Peptides of p53 C-Terminus; Long-term Inhibitory Effect on the Growth of Bladder Cancer Urology 75, 813-819 (2010).
  56. NAKASE, Ikuhiko.; KOGURE, Kentaro.; HARASHIMA, Hideyoshi.; FUTAKI, Shiroh., Chapter 37. Application of a Fusiogenic Peptide GALA for Intracellular Delivery In Cell-Penetrating Peptides; Methods and Protocols Ed. By Ülo Langel Methods in Molecular Biology, Springer 683, 139-144 (2010).
  57. El-Sayed A, Futaki S, Harashima H, Delivery of Macromolecules using Arginine-rich Cell Penetrating Peptides; Ways to Overcome Endosomal Entrapment AAPS J. 11(1), 13-22 (2009).
  58. Shiga K, Takayama K, Futaki S, E. Hutti J, Cantley L C , Ueki K, Ono Y, Mukai H, Development of an Intracellularly-Acting Inhibitory Peptide Selective for PKN Biochem. J. 425(2), 445-453 (2009).
  59. El-Sayed A, Futaki S, Harashima H, Delivery of Macromolecules using Arginine-rich Cell Penetrating Peptides; Ways to Overcome Endosomal Entrapment AAPS J. 11(1), 13-22 (2009).
  60. Guterstam P, Madani F, Hirose H, Takeuchi T, Futaki S, EL-Andaloussi S, Graslund A, Langel U, Elucidating Cell-Penetrating Peptide Mechanisms of Action for Membrane Interaction, Cellular Uptake, and Translocation Utilizing the Hydrophobic Counter-Anion Pyrenebutyrate Biochim. Biophys. Acta. - Biomembranes 1788(12), 2509-2517 (2009).
  61. Nishino A, Sawa N, Onodera K, Sakamoto S, Araki Y, Nakase I, Futaki S, Inoue Y, Wada T, Studies on the Effects of Arginine Residues Introduced to Peptide Ribonucleic Acids (PRNA) on the Complex Stability with RNA Nucleic Acids Symp. Ser. (53), 55-56 (2009).
  62. Nakase I, Hirose H, Tanaka G, Tadokoro A, Kobayashi S, Takeuchi T, Futaki S, Cell Surface Accumulation of Flock House Virus-derived Peptide Leads to Efficient Internalization via Macropinocytosis Mol. Ther. 17(11), 1868-1876 (2009).
  63. Imanishi M, Nakamura A, Morisaki T, Futaki S, Positive and Negative Cooperativity of Modularly Assembled Zinc Fingers Biochem. Biophys. Res. Commun. 387(3), 440-443 (2009).
  64. Kurihara D, Akita H, Kudo A, Masuda T, Futaki S, Harashima H, Effect of Polyethyleneglycol Spacer on the Binding Properties of Nuclear Localization Signal-Modified Liposomes to Isolated Nucleus Biol. Pharm. Bull. 32(7), 1303-1306 (2009).
  65. Hatakeyama H, Itho E, Akita H, Oishi M, Nagasaki Y, Futaki S, Harashima H, A pH-sensitive Fusogenic Peptide Facilitates Endosomal Escape and Greatly Enhances the Gene Silencing of siRNA-containing Nanoparticles in vitro and in vivo J. Control. Release 139(2), 127-132 (2009).
  66. Azuma Y, Imanishi M, Yoshimura T, Kawabata T, Futaki S, Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid Angew. Chem. Int. Ed. 48(37), 6853-6856 (2009).
  67. Takayama K, Nakase I, Michiue H, Takeuchi T, Tomizawa K, Matsui H, Futaki S, Enhanced Intracellular Delivery Using Arginine-Rich Peptides by the Addition of Penetration Accelerating Sequences (Pas) J. Control. Release 138(2), 128-133 (2009).
  68. Sakurai Y, Hatakeyama H, Akita H, Oishi M, Nagasaki Y, Futaki S, Harashima H, Efficient Short Interference RNA Delivery to Tumor Cells Using a Combination of Octaarginine, GALA and Tumor-Specific, Cleavable Polyethylene Glycol System Biol. Pharm. Bull. 32(5), 928-932 (2009).
  69. Watkins C L, Brennan P, Fegan C, Takayama K, Nakase I, Futaki S, Jones A T, Cellular Uptake, Distribution and Cytotoxicity of the Hydrophobic Cell Penetrating Peptide Sequence PFVYLI Linked to the Proapoptotic Domain Peptide PAD J. Control. Release 140(3), 237-244 (2009).
  70. Kobayashi S, Nakase I, Kawabata N, Yu H, Pujals S, Imanishi M, Giralt E, Futaki S, Cytosolic Targeting of Macromolecules Using a pH-dependent Fusogenic Peptide in Combination with Cationic Liposomes Bioconjug. Chem. 20(5), 953-959 (2009).
  71. Gao C, Izquierdo-Barba I, Nakase I, Futaki S, Ruan J, Sakamoto K, Sakamoto Y, Kuroda K, Terasaki O, Shunai Che, Mesoporous Silica Based Delivery System for a Drug with a Peptide as a Cell-Penetrating Vector Microporous and Mesoporous Materials 122(1-3), 201-207 (2009).
  72. Watkins C L, Schmaljohann D, Futaki S, Jones A T, Low Concentration Thresholds of Plasma Membranes for Rapid Energy-Independent Translocation of a Cell Penetrating Peptide Biochem. J. 420(2), 179-189 (2009).
  73. Inomata K,Ohno A, Tochio H, Isogai S, Tenno T, Nakase I, Takeuchi T, Futaki S, Ito Y, Hiroaki H, Shirakawa M, High-resolution Multi-dimensional NMR Spectroscopy of Proteins in Human Cells Nature 458(7234), 106-110 (2009).
  74. Takayama K, Tadokoro A, Pujals S, Nakase I, Giralt E, and Futaki S, Novel System to Achieve One-Pot Modification of Cargo Molecules with Oligoarginine Vectors for Intracellular Delivery Bioconjug. Chem. 20(2), 249-257 (2009).
  75. Nakase I, Gallis B, Takatani-Nakase T, Oh S, Lacoste E, Singh N P, Goodlett D R,Tanaka S, Futaki S, Lai H, Sasaki T, Transferrin Receptor-dependent Cytotoxicity of Artemisinin Transferrin Conjugates on Prostate Cancer Cells and Induction of Apoptosis Cancer Lett 274(2), 290-298 (2009).
  76. Futaki S, Asami K, Ligand-Induced Extramembrane Conformation Switch Controlling Alamethicin Assembly and the Channel Current Peptaibols; Fungal Peptides Containing a-Dialkyl a-Amino Acids , 344-352 (2009).
  77. Kamada M, Maegawa M, Maeda N, Yamamoto S, Yoshikawa S, Mori H, Tanaka Y, Okada M, Shitukawa K, Irahara1 M, Aono T, Kido H, Ogushi F , Sone S, Kanayama N, Kagawa S, Ohmoto Y, Futaki S, Zhi Guo Liang, Samuel S. Koide, Immunoglobulin binding factor in human seminal plasma and uterine cerivical mucus Current Paradigm of Reproductive Immunology , 95-104 (2009).
  78. Wada T, Sawa N, Sato H, Futaki S, Inoue Y, Effects of arginine residue introduction upon interaction and complexation behavior of peptide ribonucleic acids (PRNAs) with RNA; synthesis and properties of alpha-containing arginine. Nucleic Acids Symp. Ser. (52),673-674 (2008).
  79. Takayama K, Suehisa Y, Fujita T, Nguyen J-T , Futaki S, Yamamoto A, Kiso Y, Hayashi Y, Oligoarginine-Based Prodrugs with Self-Cleavable Spacers for Caco-2 Cell Permeation Chem. Pharm. Bull. 56(11), 1515-1520 (2008).
  80. Morisaki T, Imanishi M, Futaki S, Sugiura Y, Rapid Transcriptional Activity in Vivo and Slow DNA Binding in Vitro by Artificial Multi-Zinc Finger Protein" Biochemistry 47(38), 10171-10177 (2008).
  81. El-Sayed A, Khalil I A, Kogure K, Futaki S, Harashima H, Octaarginine- and Octalysine-Modified Nanoparticles Have Different Modes of Endosomal Escape J. Biol. Chem. 283(34), 23450-23461 (2008).
  82. Nakase I, Takeuchi T, Tanaka G, Futaki S, Methodological and Cellular Aspects that Govern the Internalization Mechanisms of Arginine-rich Cell-penetrating Peptides Adv. Drug Deliv. Rev. 60(4-5), 598-607 (2008).
  83. Futaki S, Membrane Permeable Peptide Vectors; Chemistry and Functional Design for the Therapeutic Applications Adv. Drug Deliv. Rev. 60(4-5), 447 (2008).
  84. Khalil I A, Kogure K, Futaki S, Harashima H, Octaarginine-modified Liposomes; Enhanced Cellular Uptake and Controlled Intracellular Trafficking Int. J. Pharm. 354(1-2), 39-48 (2008).
  85. Kosuge M, Takeuchi T, Nakase I, Jones A T, Futaki S, Cellular Internalization and Distribution of Arginine-Rich Peptides as a Function of Extracellular Peptide Concentration, Serum, and Plasma Membrane Associated Proteoglycans Bioconjug. Chem. 19(3), 656-664 (2008).
  86. Sasaki K, Kogure K, Chaki S, Nakamura Y, Moriguchi R, Hamada H, Danev R, Nagayama K, Futaki S, Harashima H, An Artificial Virus-like Nano Carrier System; Enhanced Endosomal escape of Nanoparticles via Synergistic Action of pH-sensitive Fusogenic Peptide Derivatives Anal. Bioanal. Chem. , (2008).
  87. Futaki S, Asami K, Ligand-Induced Extramembrane Conformation Switch Controlling Alamethicin Assembly and the Channel Current Chem. Biodiv. 4(6), 1313-1322 (2007).
  88. Futaki S, Nakase I, Tadokoro A, Takeuchi T, Jones A T, Arginine-rich peptides and their internalization mechanisms Biochem. Soc. Trans. 35(Pt 4), 784-787 (2007).
  89. Fretz M M, Penning N A, Al-Taei S, Futaki S, Takeuchi T, Nakase I, Storm G, Jones A T, Temperature, Concentration and Cholesterol Dependent Translocation of L- and D-octaarginine across the Plasma and Nuclear membrane of CD34 +  Leukaemia Cells Biochemical J. 403(2), 335-342 (2007).
  90. Kameyama S, Horie M, Kikuchi T, Omura T, Tadokoro A, Takeuchi T, Nakase I, Sugiura Y, Futaki S, The Use of Acid Wash in Determining Cellular Uptake of Fab/Cell-Permeating Peptide Conjugates Biopolymers (Peptide Science) 88(2), 98-106 (2007).
  91. Khalil I A1, Kogure K, Futaki S, Hama S, Akita H, Ueno M, Kishida H,Kudoh M, Mishina Y, Kataoka K, Yamada M, Harashima H, Octaarginine-modified Multifunctional Envelope-type Nanoparticles for Gene Delivery Gene Ther. 14(8), 682-689 (2007).
  92. Kimura T, Ninomiya K, Futaki S, NMR Investigation of the Electrostatic Effect in Binding of a Neuropeptide, Achatin-I, to Phosphatidylcholine Bilayers. J. Phys. Chem. B. 111(14), 3831-3838 (2007).
  93. Homhuan A, Kogure K, Akaza H, Futaki S, Naka T, Fujita Y, Yano I, Harashima H, New Packaging Method of Mycobacterial Cell Wall using Octaarginine-modified Liposomes; Enhanced Uptake by and Immunostimulatory Activity of Dendritic Cells J. Control. Release 120(1-2), 60-69 (2007).
  94. Nakamura Y, Kogure K, Futaki S, Harashima H, Octaarginine-modified Multifunctional Envelope-type Nano Device for siRNA J. Control. Release 119(3), 360-367 (2007).
  95. Yan W, Imanishi M, Futaki S, Sugiura Y, α-Helical Linker of an Artificial 6-Zinc Finger Peptide Contributes to Selective DNA Binding to a Discontinuous Recognition Sequence Biochemistry 46(29), 8517-8524 (2007).
  96. Maiti K K, Woo Sirl Lee, Takeuchi T, Watkins C, Fretz M, Kim D-C, Futaki S, Jones A, Kim K-T, Chung S-K, Guanidine-Containing Molecular Transporters; Sorbitol-Based Transporters Show High Intracellular Selectivity toward Mitochondria Angew. Chem. Int. Ed. Engl. 46(31), 5880-5884 (2007).
  97. Hayashi Y, Takayama K, Suehisa Y, Fujita T, Nguyen J-T, Futaki S, Yamamoto , Kiso Y, Development of Oligoargininedrug Conjugates Linked to New Peptidic Self-cleavable Spacers toward Effective Intestinal Absorption Bioorg. Med. Chem. Lett. 17(18), 17(18) (2007).
  98. Noguchi H, Nakai Y, Ueda M, Masui Y, Futaki S, Kobayashi N, Hayashi S, Matsumoto S, Activation of JNK pathway during islet transplantation and prevention of islet graft loss by intraportal injection of JNK inhibitor Diabetologia 50 , 612-619 (2007).
  99. Nakase I, Tadokoro A, Kawabata N, Takeuchi T,Katoh H, Hiramoto K, Negishi M, Nomizu M, Sugiura Y, Futaki S, Interaction of Arginine-Rich Peptides with Membrane-Associated Proteoglycans Is Crucial for Induction of Actin Organization and Macropinocytosis Biochemistry46 , 492-501 (2007).
  100. Wada T, Maeda Y, Sawa N, Sato H, Futaki S, Inoue Y, RNA Recognition and Recognition Control of α-Peptide Ribonucleic Acids Containing Arginine Residue by External Factors Nucleic Acids Symp. Ser.50 , 75-76 (2006).
  101. Sakai N, Futaki S, Matile S, Anion Hopping of (and on) Functional Oligoarginines; From Chloroform to Cells Soft Matter2 , 636 - 641 (2006).
  102. Futaki S, Oligoarginine Vectors for Intracellular Delivery; Design and Cellular-uptake Mechanisms Biopolymers (Peptide Science)84 , 241-249 (2006).
  103. 二木史朗, ペプチドによる細胞内移送制御 高分子55 , 345 (2006).
  104. Otani Y, Futaki S, Kiwada T, Sugiura Y, Muranaka A, Kobayashi N, Uchiyama M, Yamaguchi K, Ohwada T, Oligomers of β-Amino Acid Bearing Non-planar Amides Form Ordered Structures Tetrahedron62 , 11635-11644 (2006).
  105. Fretz M, Jin J, Conibere R, Penning N A, Taei S A, Storm G, Futaki S, Takeuchi T, Nakase I, Jones A T, Effects of Na+/H+ Exchanger Inhibitors on Subcellular Localisation of Endocytic Organelles and Intracellular Dynamics of Protein Transduction Domains HIV-TAT Peptide and Octaarginine J. Control. Release116 , 247-254 (2006).
  106. Takeuchi T, Kosuge M, Tadokoro A, Sugiura Y, Nishi M, Kawata M, Sakai N, Matile S, Futaki S, Direct and Rapid Cytosolic Delivery Using Cell-Penetrating Peptides Mediated by Pyrenebutyrate ACS Chem. Biol.1 , 299-303 (2006).
  107. Kameyama S, Horie M, Kikuchi T, Omura T, Takeuchi T, Nakase I, Sugiura Y, Futaki S, Effects of Cell-Permeating Peptide Binding on the Distribution of 125I-Labeled Fab Fragment in Rats Bioconjug. Chem.17 , 597-602 (2006).
  108. Taei S A, Penning N A, Simpson J C, Futaki S, Takeuchi T, Nakase I, Jones A T, Intracellular Traffic and Fate of Protein Transduction Domains HIV-1 TAT Peptide and Octaarginine. Implications for their Utilization as Drug Delivery Vectors Bioconjug. Chem.17 , 90-100 (2006).
  109. Khalil I A, Kogure K, Futaki S, Harashima H, High Density of Octaarginine Stimulates Macropinocytosis Leading to Efficient Intracellular Trafficking for Gene Expression J. Biol. Chem.281 , 3544-3551 (2006).
  110. Kameyama S,Okada R, Kikuchi T, Omura T,Nakase I, Takeuchi T, Sugiura Y, Futaki S, Distribution of Immunoglobulin Fab Fragment Conjugated with HIV-1 REV Peptide Following Intravenous Administration in Rats Mol. Pharm.3 , 174-180 (2006).
  111. Mudhakir D, Akita H, Khalil I A, Futaki S, Harashima H, Pharmacokinetic Analysis of the Tissue Distribution of Octaarginine Modified Liposomes in Mice Drug Metab. Pharmacokinet.20 , 275-281 (2005).
  112. Yamada Y, Shinohara Y, Kakudo T, Chaki S, Futaki S, Kamiya H, Harashima H, Mitochondrial Delivery of Mastoparan with Transferrin Liposomes Equipped with a pH-sensitive Fusogenic Peptide for Selective Cancer Therapy Int. J. Pharm. 303, 1-7 (2005).
  113. Moriguchi R, Kogure K , Akita H, Futaki S, Miyagishi M, Taira K, Harashima H, A Multifunctional Envelope-type Nano Device for Novel Gene Delivery of siRNA Plasmids. Int. J. Pharm.301 , 277-85 (2005).
  114. Noguchi H, Nakai Y, Matsumoto S, Kawaguchi M, Ueda M, Okitsu T, Iwanaga Y, Yonekawa Y, Nagata H, Minami K, Masui Y, Futaki S, Tanaka K., Cell permeable peptide of JNK inhibitor prevents islet apoptosis immediately after isolation and improves islet graft function. Am J Transplant 5, 1848-1855 (2005).
  115. Futaki S, Masui Y, Nakase I, Sugiura Y, Nakamura T, Kogure K, Harashima H., Unique features of a pH-sensitive fusogenic peptide that improves the transfection efficiency of cationic liposomes. J. Gene. Med. 7, 1450-1458 (2005).
  116. Perret F, Nishihara M, Takeuchi T, Futaki S, Lazar A N, Coleman A, Sakai N, Matile S, Anionic Fullerenes, Calixarenes, Coronenes, and Pyrenesas Activators of Oligo/Polyarginines in Model Membranes and Live Cells J. Am. Chem. Soc.127, 1114-1115 (2005).
  117. Nakatsukasa T, Shiraishi Y, Negi S, Imanishi M, Futaki S, Sugiura Y, Site-Specific DNA Cleavage by Artificial Zinc Finger-Type Nuclease with Cerium-Binding Peptide Biochem. Biophys. Res. Commun. 330, 247-252 (2005).
  118. Nakase I, Niwa M, Takeuchi T, Sonomura K, Kawabata N, Koike Y, Takehashi M, Tanaka S, Ueda K, Simpson C. J, Jones T. A, Sugiura Y, Futaki S, Cellular Uptake of Arginine-Rich Peptides; Roles for Macropinocytosis and Actin Rearrangement Mol. Ther. 10(6), 1011-1022 (2004).
  119. Futaki S, Kiwada T, Sugiura Y, Control of Peptide Structure and Recognition by Fe(III)-induced Helix Destabilization J. Am. Chem. Soc. 126(48), 15762-15769 (2004).
  120. Kogure K, Moriguchi R, Sasaki K, Ueno M, Futaki S, Harashima H, Development of a Non-viral Multifunctional Envelop-type Nano Device by a Novel Lipid Film Hydration Method J. Control. Release 98, 317-323 (2004).
  121. Futaki S, Niwa M, Nakase I, Tadokoro A, Zhang Y, Nagaoka M, Wakako N, Sugiura Y, Arginine Carrier Peptide Bearing Ni(II) Chelator to Promote Cellular Uptake of Histidine-Tagged Proteins Bioconjug. Chem. 15, 475-481 (2004).
  122. Akita H, Ito R, Khalil A. I, Futaki S, Harashima H, Quantitative Three-dimensional Analysis of the Intracellular Trafficking of Plasmid DNA Transfected by Non-viral Gene Delivery System using Confocal Laser Scanning Microscopy Mol. Ther. 9, 443-451 (2004).
  123. Futaki S, Kiwada T, Shiraishi Y, Sugiura Y, Total Synthesis of Artificial Zinc Finger Proteins; Problems and Perspectives Biopolymers (Peptide Science) 76, 98-109 (2004).
  124. Ohwada T, Kojima D, Kiwada T, Futaki S, Sugiura Y, Yamaguchi K, Nishi Y, bayashi Y, α,α-Disubstituted Glycines Bearing a Large Hydrocarbon Ring. Peptide Self-Assembly through Novel Hydrophobic Recognition Chemistry- A Eur J. 10, 617-626 (2004).
  125. Kitagawa K, Adachi H, Sekigawa Y, Yagami T, Futaki S, Gu Jun Yuan, Inoue K, Total Synthesis of Large CCK Isomers Using a Thioester Segment Condensation Approach Tetrahedron 60, 907-918 (2004).
  126. Khalil A. I, Futaki S, Niwa M, Baba Y, Kaji N, Kamiya H, Harashima H, Mechanism of Improved Gene Transfer by the N-Terminal Stearylation of Octaarginine; Enhanced Cellular association by Hydrophobic Core Formation Gene Ther. 11, 636-644 (2004).
  127. Futaki S, Zhang Y, Kiwada T, Nakase I, Yagami T, Oiki S, Sugiura Y, Gramicidin-based Channel Systems for the Detection of Protein-ligand Interaction Bioorg. Med. Chem. 12, 1343-1350 (2004).
  128. Kakudo T, Chaki S, Futaki S, Nakase I, Akaji K, Kawakami T, Maruyama K, Kamiya H, Harashima H, Transferrin-modified Liposomes Equipped with a pH-sensitive Fusogenic Peptide; an Artificial Viral-like Delivery System Biochemistry 43, 5618-5628 (2004).
  129. Futaki S, Intracellular Delivery Using Arginine Peptides J. Phar. Sci. Tech., Jpn 64, 164-167 (2004).
  130. Futaki S, Peptide-mediated Drug Delivery The Cell 36, 428-431 (2004).
  131. Futaki S, Intracellular Delivery of Proteins and Macromolecules Using Carrier Peptides Chemistry Today Aug, 32-36 (2004).
  132. Nakase I, Futaki S, Intracellular Delivery of Proteins and Drugs Using Arginine Peptides Chemistry Nov, 68-69 (2004).
  133. Nagasaki T, Myohoji T, Tachibana T, Futaki S, Tamagaki S, Can Nuclear Localization Signals Enhance Nuclear Localization of Plasmid DNA? Bioconjug. Chem. 142(2), 282-286 (2003).
  134. Tachibana R , Futaki S, Harashima H, Kiwada H, pH-sensitive Liposomes in Nuclear Targeting of Macromolecules Methods Enzymol. 372, 349-361 (2003).
  135. Shibata A, Murata S, Ueno S, Shaoqian Liu, Futaki S, Baba Y, Synthetic Copoly(Lys/Phe) and Poly(Lys) Translocate through Lipid Bilayer Membranes Biochim. Biophys, Acta - Biomembranes 1616(), 147-155 (2003).
  136. Futaki S, Current Protein & Peptide Science 4巻2号, (2003).
  137. Yano Y, Takemoto T, Kobayashi S, Yasui H, Sakurai H, Ohashi W, Niwa M, Futaki S, Sugiura Y, Matsuzaki K, Topological Stability and Self-Association of a Completely Hydrophobic Model Transmembrane Helix in Lipid Bilayers Biochemistry 41, 3073-3080 (2002).
  138. Futaki S, Nakase I, Suzuki T, Zhang Y, Sugiura Y, Translocation of Branched-Chain Arginine Peptides through Cell Membranes; Flexibility in the Spatial Disposition of Positive Charges in Membrane-Permeable Peptides Biochemistry 41, 7925-7930 (2002).
  139. Zhang Y, Futaki S, Kiwada T, Sugiura Y, Detection of Protein-Ligand Interaction on the Membranes Using C-Terminus Biotin-Tagged Alamethicin Bioorg. Med. Chem. 10, 2635-2639 (2002).
  140. Suzuki T, Futaki S, Niwa M, Tanaka S, Ueda K, Sugiura Y, Possible Existence of Common Internalization Mechanisms among Arginine-rich Peptides J. Biol. Chem. 277, 2437-2443 (2002).
  141. Okumura Y, Kamikubo Y, Curriden S A, Wang J, Kiwada T, Futaki S, Kitagawa K, Loskutoff D J, Kinetic Analysis of the Interaction between Vitronectin and the Urokinase Receptor J. Biol. Chem. 277, 9395-9404 (2002).
  142. Futaki S, Arginine-Rich Peptides; Potential for Intracellular Delivery of Macromolecules and the Mystery of the Translocation Mechanisms Int. J. Pharmaceutics 245, 1-7 (2002).
  143. Suzuki T, Futaki S, Niwa M, Nakase I, Tanaka S, Ueda K, Sugiura Y, Involvement of Sulfated Glycosaminoglycans with the Internalization of Basic Peptides Peptide Science2001, ed by Aoyagi H The Japanese Peptide Society, 221-223 (2002).
  144. Futaki S, Membrane Permeable Basic Peptides; The Potential of these Peptides for theIntracellular Delivery of Proteins and Drugs Tanpakushitsu Kakusan Koso 47, 1415-1419 (2002).
  145. Tamiya E, Futaki S, Yoshida K, Future of combinatorial Bioengineering Chemistry Today 372, 23-30 (2002).
  146. Tanaka D, Takehashi M, Matoh N, Iida S, Suzuki T, Futaki S, Hamada H, Masliah E, Sugiura Y, Ueda K, Generation of Reactive Oxygen Species and Activation of NF-kB by non-Ab Component of Alzheimer's Disease Amyloid J. Neurochem. 82, 305-315 (2002).
  147. Takikawa M, Kamada M, Maegawa M, Yamano S, Irahara M, Aono T, Futaki S, Ohmoto Y, Koide S S, Evaluation of Two Sperm Antigens, rsmp-B and Ywk-Ii, as Targets for Immunocontraception Zygote 9, 145-151 (2001).
  148. Futaki S, Zhang Y J, Sugiura Y, Detecting a Tag on a Channel Opening; Blockage of the Biotinylated Channels by Streptavidin Tetrahedron Lett. 42, 1563-1565 (2001).
  149. Futaki S, Fukuda M, Omote M, Yamauchi K, Yagami T, Niwa M, Sugiura Y, Alamethicin-Leucine Zipper Hybrid Peptide; A Prototype for the Design of Artificial Receptors and Ion Channels J. Am. Chem. Soc. 123, 12127-12134 (2001).
  150. Kitagawa K, Aida C, Fujiwara H, Yagami T, Futaki S, Kogire M, Ida J, Inoue K, Facile Solid-Phase Synthesis of Sulfated Tyrosine-Containing Peptides; Total Synthesis of Human Big Gastrin-Iiand Cholecystokinin (Cck)-39(1,2) J. Org. Chem. 66, 1-10 (2001).
  151. Futaki S, Suzuki T, Ohashi W, Yagami T, Tanaka S, Ueda K, Sugiura Y, Arginine-Rich Peptides - an Abundant Source of Membrane-Permeable Peptides Having Potential as Carriers for Intracellular Protein Delivery J. Biol. Chem. 276, 5836-5840 (2001).
  152. Kitagawa K, Aida C, Fujiwara H, Yagami T, Futaki S, Facile Solid-Phase Synthesis of Sulfated Tyrosine-Containing Peptides; Part II. Total Synthesis of Human Big Gastrin-Iiand Its C-Terminal Glycine-Extended Peptide (G34-Gly Sulfate) by the Solid-Phase Segment Condensation Approach Chem. Pharm. Bull. 49, 958-963 (2001).
  153. Futaki S, Araki M, Kiwada T, Nakase I, Sugiura Y, A 'Cassette' Rnase; Site-Selective Cleavage of Rnaby RNase S Equipped with Rna-Recognition Segment Bioorg. Med. Chem. Lett. 11, 1165-1168 (2001).
  154. Futaki S, Ohashi W, Suzuki T, Niwa M, Tanaka S, Ueda K, Harashima H, Sugiura Y, Stearylated Arginine-Rich Peptides; A New Class of Transfection Systems Bioconjugate Chem. 12, 1005-1011 (2001).
  155. Arakaki N, Ueyama Y, Hirose M, Himeda T, Shibata H, Futaki S, Kitagawa K, Higuti T, Stoichiometry of Subunit E in Rat Liver Mitochondrial H+-Atp Synthase and Membrane Topology of Its Putative Ca2+-Dependent Regulatory Region Biochim. Biophys. Acta-Bioenerg. 1504, 220-228 (2001).
  156. Futaki S, Assembly Modulation of Channel-Forming Peptides Self-Assembling Peptide Systems in Biology, Medicine and Engineering , 87-104 (2001).
  157. Hori Y, Suzuki K, Okuno Y, Nagaoka M, Futaki S, Sugiura Y, Artificial Zinc Finger Peptide Containing a Novel His4 Domain J. Am. Chem. Soc. 122, 7648-7653 (2000).
  158. Yagami T, Kitagawa K, Aida C, Fujiwara H, Futaki S, Stabilization of a Tyrosine O-Sulfate Residue by a Cationic Functional Group; Formation of a Conjugate Acid-Base Pair J. Pept. Res. 56, 239-249 (2000).
  159. Monoi H, Futaki S, Kugimiya S, Minakata H, Yoshihara K, Poly-L-Glutamine Forms Cation Channels; Relevance to the Pathogenesis of the Polyglutamine Diseases Biophys. J. 78, 2892-2899 (2000).
  160. Kamada M, Yamamoto S, Takikawa M, Kunimi K, Maegawa M, Futaki S, Ohmoto Y, Aono T, Koide S S, Identification of the Human Sperm Protein That Interacts with Sperm-Immobilizing Antibodies in the Sera of Infertile Women Fertil. Steril. 72, 691-695 (1999).
  161. Futaki S, Aoki M, Ishikawa T, Kondo F, Asahara T, Niwa M, Nakaya Y, Yagami T, Kitagawa K, Chemical Ligation to Obtain Proteins Comprising Helices with Individual Amino Acid Sequences Bioorg. Med. Chem. 7, 187-192 (1999).
  162. Futaki S, Peptide Synthesis Aiming at Elucidation and Creation of Protein Functions Yakugaku Zasshi J. Pharm. Soc. Jpn. 118, 493-510 (1998).
  163. Futaki S, Creation of Ion Channel Function Using Synthetic Peptides J. Synth. Org. Chem. Jpn. 56, 125-133 (1998).
  164. Futaki S, Peptide Ion Channels; Design and Creation of Function Biopolymers 47, 75-81 (1998).
  165. Kamada M, Mori H, Maeda N, Yamamoto S, Kunimi K, Takikawa M, Maegawa M, Aono T, Futaki S, Koide S S, b-Microseminoprotein Prostatic Secretory Protein Is a Member of Immunoglobulin Binding Factor Family Biochim. Biophys. Acta-Protein Struct. Molec. Enzym. 1388, 101-110 (1998).
  166. Tachibana R, Harashima H, Shono M, Azumano M, Niwa M, Futaki S, Kiwada H, Intracellular Regulation of Macromolecules Using pH-Sensitive Liposomes and Nuclear Localization Signal; Qualitative and Quantitative Evaluation of Intracellular Trafficking Biochem. Biophys. Res. Commun. 251, 538-544 (1998).
  167. Mori H, Kamada M, Maegawa M, Yamamoto S, Aono T, Futaki S, Yano M, Kido H, Koide S S, Enzymatic Activation of Immunoglobulin Binding Factor in Female Reproductive Tract Biochem. Biophys. Res. Commun. 246, 409-413 (1998).
  168. Tsutsumishita Y, Onda T, Okada K, Takeda M, Endou H, Futaki S, Niwa M, Involvement of H2o2 Production in Cisplatin-Induced Nephrotoxicity Biochem. Biophys. Res. Commun. 242, 310-312 (1998).
  169. Futaki S, Aoki M, Fukuda M, Kondo F, Niwa M, Kitagawa K, Nakaya Y, Assembling of the Four Individual Helices Corresponding to the Transmembrane Segments (S4 in Repeat I-Iv) of the Sodium Channel Tetrahedron Lett. 38, 7071-7074 (1997).
  170. Futaki S, Sogawa K, Maruyama J, Asahara T, Niwa M, Hojo H, Preparation of Peptide Thioesters Using Fmoc-Solid-Phase Peptide Synthesis and Its Application to the Construction of a Template-Assembled Synthetic Protein (Tasp) Tetrahedron Lett. 38, 6237-6240 (1997).
  171. Kitagawa K, Aida C, Fujiwara H, Yagami T, Futaki S, Efficient Solid-Phase Synthesis of Sulfated Tyrosine-Containing Peptides Using 2-Chlorotrityl Resin; Facile Synthesis of Gastrin/Cholecystokinin Peptides Tetrahedron Lett. 38, 599-602 (1997).
  172. Futaki S, Kitagawa K, Peptide-Unit Assembling Via Disulfide Cross-Linking; A Versatile Approach Which Enables the Creation of Artificial Proteins Comprising Helices with Different Amino Acid Sequences Tetrahedron 53, 7479-7492 (1997).
  173. Niwa M, Kawai Y, Nakamura N, Futaki S, The Structure of the Promoter Region for Rat Inducible Nitric Oxide Synthase Gene Life Sci. 61, PL45-PL49 (1997).
  174. Kitagawa K, Mizobuchi N, Hama T, Hibi T, Konishi R, Futaki S, Synthesis and Antinociceptive Activity of [D-Ala2]Leu- Enkephalin Derivatives Conjugated with the Adamantane Moiety Chem. Pharm. Bull. 45, 1782-1787 (1997).
  175. Futaki S, Ishikawa T, Niwa M, Kitagawa K, Yagami T, Embodying a Stable Alpha-Helical Protein Structure through Efficient Chemical Ligation Via Thioether Formation Bioorg. Med. Chem. 5, 1883-1891 (1997).
  176. Niwa M, Nakamura N, Kitajima K, Ueda M, Tsutsumishita Y, Futaki S, Takaishi Y, Benzoquinones Inhibit the Expression of Inducible Nitric Oxide Synthase Gene Biochem. Biophys. Res. Commun. 239, 367-371 (1997).
  177. Futaki S, Ishikawa T, Niwa M, Yagami T, Kitagawa K, Pseudo-Symmetrical Protein; Construction of a Four-Helix-Bundle Protein with Four Intramolecular Disulfide Cross-Linkings Tetrahedron Lett. 37, 201-204 (1996).
  178. Yokoi H, Tsuruo Y, Miyamoto T, Kitagawa K, Futaki S, Ishimura K, Steroid 5a-Reductase Type 1 Immunolocalized in the Anterior Pituitary of Intact and Castrated Male Rats Histochem. Cell Biol. 106, 359-366 (1996).
  179. Miyamoto T, Kagawa S, Kitagawa K, Futaki S, Yokoi H, Tsuruo Y, Ishimura K, Immunocytochemical Localization of 5a-Reductase Type 1 in the Prostate of Normal and Castrated Rats Histochem. Cell Biol. 105, 101-109 (1996).
  180. Niwa Y, Yano M, Futaki S, Okumura Y, Kido H, T-Cell Membrane-Associated Serine Protease, Tryptase Tl2, Binds Human Immunodeficiency Virus Type 1 Gp120 and Cleaves the Third-Variable-Domain Loop of Gp120-Neutralizing Antibodies of Human Immunodeficiency Virus Type 1 Inhibit Cleavage of Gp1 Eur. J. Biochem. 237, 64-70 (1996).
  181. Tsuruo Y, Miyamoto T, Yokoi H, Kitagawa K, Futaki S, Ishimura K, Immunohistochemical Presence of 5a-Reductase Rat Type 1-Containing Cells in the Rat Brain Brain Res. 722, 207-211 (1996).
  182. Tsutsumishita Y, Kawai Y, Takahara H, Onda T, Miyoshi J, Futaki S, Niwa M, Sequence Analysis of Inducible Nitric Oxide Synthase in Rat Kidney, Lung, and Uterus Biol. Pharmacol. Bull. 19, 1374-1376 (1996).
  183. Niwa M, Tsutsumishita Y, Kawai Y, Takahara H, Nakamura N, Futaki S, Takaishi Y, Kondoh W, Moritoki H, Suppression of Inducible Nitric Oxide Synthase Mrna Expression by Tryptoquinone A Biochem. Biophys. Res. Commun. 224, 579-585 (1996).
  184. Futaki S, Ishikawa T, Niwa M, Kitagawa K, Yagami T, Peptide-Unit Assembling Using S-Alkylation - a Highly Denaturation Resistant Protein Was Obtained Tetrahedron Lett. 36, 5203-5206 (1995).
  185. Yagami T, Kitagawa K, Futaki S, Liquid Secondary-Ion Mass-Spectrometry of Peptides Containing Multiple Tyrosine-O-Sulfates Rapid Commun. Mass Spectrom. 9, 1335-1341 (1995).
  186. Yagami T, Kitagawa K, Futaki S, Analysis of Sulfated Tyrosine-Containing Peptides by Liquid Secondary-Ion Mass-Spectrometry with Constant Neutral-Loss (80 Amu) Scanning Anal. Sci. 11, 1025-1028 (1995).
  187. Futaki S, Kitagawa K, Peptide-Unit Assembling Using Disulfide Cross-Linking - a New Approach for Construction of Protein Models Tetrahedron Lett. 35, 1267-1270 (1994).
  188. Maeda N, Kamada M, Daitoh T, Maegawa M, Hirano K, Aono T, Shiotsu T, Kanayama H, Kagawa S, Futaki S, Muraguchi M, Ohmoto Y, Liang Z G, Koide S S, Immunoglobulin Binding-Factor - a New Tumor-Marker for Prostatic Tumors Prostate 24, 125-130 (1994).
  189. Kitagawa K, Futaki S, Yagami T, A Novel-Approach for the Synthesis of Tyrosine Sulfate-Containing Peptides Using a Safety Catch Type Protecting Group as a Key Feature J. Synth. Org. Chem. Jpn. 52, 675-685 (1994).
  190. Tsuzuki N, Hama T, Kawada M, Hasui A, Konishi R, Shiwa S, Ochi Y, Futaki S, Kitagawa K, Adamantane as a Brain-Directed Drug Carrier for Poorly Absorbed Drug. 2. Azt Derivatives Conjugated with the 1-Adamantane Moiety J. Pharm. Sci. 83, 481-484 (1994).
  191. Kitagawa K, Futaki S, Yagami T, Sumi S, Inoue K, Solid-Phase Synthesis of Cionin, a Protochordate-Derived Octapeptide Related to the Gastrin/Cholecystokinin Family of Peptides, and Its Mono-Tyrosine-Sulfate-Containing Derivatives Int. J. Pept. Protein Res. 43, 190-200 (1994).
  192. Kamada M, Maeda N, Maegawa M, Mori H, Yamamoto S, Aono T, Muraguchi M, Ohmoto Y, Futaki S, Ogushi F, Sone S, Kanayama H, Kagawa S, Koide S S, Detection of Immunoglobulin Binding-Factor by Enzyme-Linked- Immunosorbent-Assay Using 2 Monoclonal-Antibodies Biochem. Mol. Biol. Int. 34, 837-844 (1994).
  193. Owhashi M, Futaki S, Kitagawa K, Horii Y, Maruyama H, Hayashi H, Nawa Y, Molecular-Cloning and Characterization of a Novel Neutrophil Chemotactic Factor from a Filarial Parasite Mol. Immunol. 30, 1315-1320 (1993).
  194. Bhatia M B, Futaki S, Ueno H, Manning J M, Ringe D, Yoshimura T, Soda K, Kinetic and Stereochemical Comparison of Wild-Type and Active-Site K145q Mutant Enzyme of Bacterial D-Amino-Acid Transaminase J. Biol. Chem. 268, 6932-6938 (1993).
  195. Yagami T, Shiwa S, Futaki S, Kitagawa K, Evaluation of the Final Deprotection System for the Solid-Phase Synthesis of Tyr(So3h)-Containing Peptides with 9- Fluorenylmethyloxycarbonyl (Fmoc)-Strategy and Its Application to the Synthesis of Cholecystokinin (Cck)-12 Chem. Pharm. Bull. 41, 376-380 (1993).
  196. Maeda N, Kamada M, Daitoh T, Aono T, Futaki S, Liang Z G, Koide S S, Immunoglobulin Binding-Factor in Human Seminal Plasma - Immunological Function Arch. Androl. 31, 31-36 (1993).
  197. Futaki S, Taike T, Akita T, Kitagawa K, Syntheses of 2 Tyrosine Sulfate Containing Peptides, Leucosulfakinin (Lsk)-Iiand Cholecystokinin (Cck)-12, Using the O-Para-(Methylsulphinyl)Benzyl Serine for the Selective Sulfation of Tyrosine Tetrahedron 48, 8899-8914 (1992).
  198. Delpozo A M, Yoshimura T, Bhatia M B, Futaki S, Manning J M, Ringe D, Soda K, Inactivation of Dimeric D-Amino-Acid Transaminase by a Normal Substrate through Formation of an Unproductive Coenzyme Adduct in One Subunit Biochemistry 31, 6018-6023 (1992).
  199. Kuraishi Y, Kawamura M, Yamaguchi T, Houtani T, Kawabata S, Futaki S, Fujii N, Satoh M, Intrathecal Injections of Galanin and Its Antiserum Affect Nociceptive Response of Rat to Mechanical, but Not Thermal, Stimuli Pain 44, 321-324 (1991).
  200. Nishimura K, Tanizawa K, Yoshimura T, Esaki N, Futaki S, Manning J M, Soda K, Effect of Substitution of a Lysyl Residue That Binds Pyridoxal-Phosphate in Thermostable D-Amino-Acid Aminotransferase by Arginine and Alanine Biochemistry 30, 4072-4077 (1991).
  201. Tsuzuki N, Hama T, Hibi T, Konishi R, Futaki S, Kitagawa K, Adamantane as a Brain-Directed Drug Carrier for Poorly Absorbed Drug-Antinociceptive Effects of [D-Ala2]Leu-Enkephalin Derivatives Conjugated with the 1-Adamantane Moiety Biochem. Pharmacol. 41, R5-R8 (1991).
  202. Hirao H, Fujita T, Iwasaki Y, Ide H, Inoue S, Kitagawa K, Futaki S, Kawanishi H, Akita T, Studies on Antiallergic Agent. 1. 1,2,3-Trisubstituted-2-Propen-1-One Derivatives, 3,4-Disubstituted-4-Oxo-2-Butenoic Acids and the Related-Compounds Yakugaku Zasshi-J. Pharm. Soc. Jpn. 110, 727-736 (1990).
  203. Futaki S, Taike T, Yagami T, Ogawa T, Akita T, Kitagawa K, Use of Dimethylformamide Sulfur-Trioxide Complex as a Sulfating Agent of Tyrosine J. Chem. Soc. Perkin Trans. 1, 1739-1744 (1990).
  204. Futaki S, Yagami T, Taike T, Akita T, Kitagawa K, Sulfur-Trioxide Thiol-a Novel System for the Reduction of Methionine Sulfoxide J. Chem. Soc. Perkin Trans. 1 , 653-658 (1990).
  205. Futaki S, Taike T, Akita T, Kitagawa K, A New Approach for the Synthesis of Tyrosine Sulfate Containing Peptides - Use of the p-(Methylsulphinyl)Benzyl Group as a Key Protecting Group of Serine J. Chem. Soc. Chem. Commun. , 523-524 (1990).
  206. Futaki S, Ueno H, Delpozo A M, Pospischil M A, Manning J M, Ringe D, Stoddard B, Tanizawa K, Yoshimura T, Soda K, Substitution of Glutamine for Lysine at the Pyridoxal-Phosphate Binding-Site of Bacterial D-Amino-Acid Transaminase-Effects of Exogenous Amines on the Slow Formation of Intermediates J. Biol. Chem. 265, 22306-22312 (1990).
  207. Kitagawa K, Tomoyasu T, Futaki S, Akita T, Higashide S, Inoue K, Tobe T, Solution Synthesis of Human Peptide-Yy(Hpyy) Chem. Pharm. Bull. 38, 1731-1734 (1990).
  208. Futaki S, Yagami T, Taike T, Ogawa T, Akita T, Kitagawa K, Facile Reduction of Methionine Sulfoxide with Sulfur-Trioxide Iodide-Ion System Chem. Pharm. Bull. 38, 1165-1169 (1990).
  209. Futaki S, Taike T, Yagami T, Akita T, Kitagawa K, Facile Reduction of Methionine Sulfoxide with Sulfur-Trioxide Thiol System Tetrahedron Lett. 30, 4411-4412 (1989).
  210. Ono S, Kiyama S, Kitagawa K, Futaki S, Nakamura T, Taike T, Akita T, Sumi S, Inoue K, Fujimura M, Tobe T, Yajima H, Application of the Trimethylsilyl Trifluoromethanesulfonate Deprotecting Procedure for the Synthesis of Porcine Peptide Yy(Pyy) Int. J. Pept. Protein Res. 33, 29-38 (1989).
  211. Kitagawa K, Kawamoto T, Futaki S, Kiyama S, Akita T, Moritoki H, Kiso Y, Solution Syntheses of 2 Enkephalin-Containing Peptides, Peptide-E and Dynorphin(1-24), Using Nin-(2,4,6- Triisopropylphenylsulfonyl)Tryptophan Chem. Pharm. Bull. 37, 2631-2638 (1989).
  212. Ono S, Kitagawa K, Futaki S, Kiyama S, Ooi Y, Akita T, Higashide S, Inoue K, Sumi S, Tobe T, Solution Synthesis of Human Neuropeptide-Y (hnpy) Chem. Pharm. Bull. 37, 1925-1929 (1989).
  213. Oda T, Futaki S, Kitagawa K, Yoshihara Y, Tani I, Higuti T, Orientation of Chargerin-Ii(A6l) in the Atp Synthase of Rat- Liver Mitochondria Determined with Antibodies against Peptides of the Protein Biochem. Biophys. Res. Commun. 165, 449-456 (1989).
  214. Iwasaki Y, Fujita T, Yabe H, Hirao H, Kitagawa K, Futaki S, Inoue T, Matsuzaki T, Akita T, Synthesis and Antifungal Activity of a Series of Novel 2- Propen-1-One Yakugaku Zasshi-J. Pharm. Soc. Jpn. 108, 942-950 (1988).
  215. Fujii N, Futaki S, Morimoto H, Inoue K, Doi R, Tobe T, Yajima H, Total Synthesis of Human Cholecystokinin-33 J. Chem. Soc. Chem. Commun. , 324-325 (1988).
  216. Fujii N, Futaki S, Funakoshi S, Akaji K, Morimoto H, Doi R, Inoue K, Kogire M, Sumi S, Yun M, Tobe T, Aono M, Matsuda M, Narusawa H, Moriga M, Yajima H, Studies on Peptides. 160. Synthesis of a 33-Residue Peptide Corresponding to the Entire Amino-Acid Sequence of Human Cholecystokinin (hcck-33) Chem. Pharm. Bull. 36, 3281-3291 (1988).
  217. Fujii N, Futaki S, Funakoshi S, Akaji K, Morimoto H, Ikemura O, Yajima H, Studies on Peptides.159. Preparation of a Protected 33-Residue Peptide for the Synthesis of Human Cholecystokinin (hCCK-33) Chem. Pharm. Bull. 36, 3271-3280 (1988).
  218. Doi R, Inoue K, Kogire M, Sumi S, Yun M, Futaki S, Fujii N, Yajima H, Tobe T, Role of Sulfated Tyrosine Residue in Influencing the Biologic Activity of Human Cholecystokinin-33 Biochem. Biophys. Res. Commun. 153, 1209-1213 (1988).
  219. Doi R, Inoue K, Kogire M, Sumi S, Yun M, Futaki S, Fujii N, Yajima H, Tobe T, Effect of Synthetic Human Cholecystokinin-33 on Exocrine Pancreas Biochem. Biophys. Res. Commun. 150, 1251-1255 (1988).
  220. Iwasaki Y, Fujita T, Yabe H, Kitagawa K, Futaki S, Matsuzaki T, Akita T, Synthesis and Antifungal Activity of Azole Derivatives of 2- Propen-1-One J. Pharm. Sci. 76, S157-S157 (1987).
  221. Robey F A, Ohura K, Futaki S, Fujii N, Yajima H, Goldman N, Jones K D, Wahl S, Proteolysis of Human C-Reactive Protein Produces Peptides with Potent Immunomodulating Activity J. Biol. Chem. 262, 7053-7057 (1987).
  222. Yajima H, Futaki S, Otaka A, Yamashita T, Funakoshi S, Bessho K, Fujii N, Akaji K, Studies on Peptides.143. Evaluation ofb-Menthylaspartate for Peptide-Synthesis Chem. Pharm. Bull. 34, 4356-4361 (1986).
  223. Fujii N, Nomizu M, Futaki S, Otaka A, Funakoshi S, Akaji K, Watanabe K, Yajima H, Studies on Peptides.132. Evaluation of 2b-Carboxyl Protecting Groups of Aspartic-Acid, Cycloheptyl and Cyclooctyl, for Peptide-Synthesis Chem. Pharm. Bull. 34, 864-868 (1986).
  224. Yajima H, Futaki S, Fujii N, Akaji K, Funakoshi S, Sakurai M, Katakura S, Inoue K, Hosotani R, Tobe T, Segawa T, Inoue A, Tatemoto K, Mutt V, Studies on Peptides.133. Synthesis and Biological-Activity of Galanin, a Novel Porcine Intestinal Polypeptide Chem. Pharm. Bull. 34, 528-539 (1986).
  225. Yajima H, Futaki S, Fujii N, Akaji K, Funakoshi S, Sakurai M, Katakura S, Inoue K, Hosotani R, Tobe T, Segawa T, Inoue A, Tatemoto K, Mutt V, Synthesis of Galanin, a New Gastrointestinal Polypeptide J. Chem. Soc. Chem. Commun. , 877-878 (1985).
  226. Fujii N, Futaki S, Akaji K, Yajima H, Inoue A, Segawa T, Studies on Peptides. 129. Application of Nin-Mesitylenesulfonyl-Tryptophan for the Syntheses of Neuromedin-B and Neuromedin-C Chem. Pharm. Bull. 33, 3731-3737 (1985).
  227. Fujii N, Hayashi Y, Futaki S, Akaji K, Yajima H, Kitagawa T, Studies on Peptides. 122. N-Succinimidyl-p-(2-Nitrovinyl)-Benzoate and Its Meta-Isomer, as Heterobifunctional Conjugating Reagents for Immunoassay Chem. Pharm. Bull. 32, 5036-5039 (1984).
  228. Fujii N, Futaki S, Yasumura K, Yajima H, Studies on Peptides. 121. Nin-Mesitylenesulfonyltryptophan, a New Derivative for Peptide-Synthesis Chem. Pharm. Bull. 32, 2660-2665 (1984).


Update: Nov 26,2014