Faculty Information, Institute for Chemical Research, Kyoto University   [ English | Japanese ]


ASAMI, Koji

Division of Multidisciplinary Chemistry - Molecular Aggregation Analysis -
Associate Professor
Doctor Science
URL:http://www003.upp.so-net.ne.jp/asami/
E-mail: asami@e.kuicr.kyoto-u.ac.jp
Tel:
Fax: 0774-38-3084
Academic career
    1977.3 Graduated from Dr. Course, Kyoto University
    1978.7 D. Sci, Kyoto University
    1979.4-1986.3 Assistant Professor, Kochi Medical School
    1986.4- Associate Professor, Kyoto University, Institute for Chemical Research
    1987.5-1989.3 Visiting Associate Professor, University of Pennsylvania (USA)
Research field
    Biophysics, Dielectrics
Current research
  1. Dielectric spectroscopy of heterogeneous systems including membranes, biological cells and colloidal particles.
  2. Ion channels formed by peptides in bilayer lipid membranes.
Selected publications
  1. Asami, K., Dielectric properties of dipicrylamine-doped erythrocytes, cultured cells and lipid vesicles Bioelectrochemistry 92, 14-21 (2013)
  2. Daoud, J.; Asami, K.; Rosenberg, L.; Tabrizian, M. , Dielectric Spectroscopy for Non-invasive Monitoring of Epithelial Cell Differentiation within Three Dimensional Scaffolds Phys. Med. Biol. 57, 5097-5112 (2012)
  3. Noshiro,D.; Asami,K.; Futaki,S., Metal-mediated Leakage Control by Histidine-containing Alamethicin Analogs Peptide Science 2011, 189-190 (2012)
  4. Noshiro, D.; Asami, K.; Futaki, S., Control of Leakage Activities of Alamethicin Analogs by Metals: Side Chain-Dependent Adverse Gating Response to Zn2+ Bioorganic & Medicinal Chemistry 20, 6870-6876 (2012)
  5. Asami, K., Dielectric Spectroscopy Reveals Nanoholes in Erythrocyte Ghosts Soft Matter 8, 3250-3257 (2012)
  6. Asami, K., Design of a Measurement Cell for Low-frequency Dielectric Spectroscopy of Biological Cell Suspensions Meas. Sci. Technol 22, 085801(7pp) (2011)
  7. Asami, K., Dielectric Properties of Microvillous Cells Simulated by the Three-dimensional Finite-element Method Bioelectrochemistry 81, 28-33 (2011)
  8. Hayashi, Y.; Katsumoto, Y.: Omori, S.; Yasuda, A.; Asami, K.; Kaibara, M.; Uchimura, I. , Dielectric Coagulometry: A New Approach to Estimate Venous Thrombosis Risk Anal. Chem. 82, 9769-9774 (2010)
  9. Asami, K., Effectiveness of "Thin-layer" and "Effective Medium" Approximations in Numerical Simulation of Dielectric Properties of Biological Cell Suspensions Jpn. J. Applied Phys. 49, 127001-1-5 (2010)
  10. Hayashi Y, Katsumoto Y, Oshige I, Omori S, Yasuda A, and Asami K, Dielectric inspection of erythrocytes J. Non-crystaline solids 356, 757-762 (2010)
  11. Noshiro D, Asami K, Futaki S, Metal-assisted channel stabilization: disposition of a single histidine on the N-terminus of alamethicin yielded channels of extraordinary long lifetimes Biophys. J 98, 1801-1808 (2010)
  12. Asami K, Simulation of dielectric spectra of erythrocytes with various shapes J. Phys. D: Appl. Phys. 42, 135503(7) (2009)
  13. Hayashi Y, Katsumoto Y, Oshige I, Omori S, Yasuda A, Asami K, The effects of erythrocyte deformability upon hematocrit assessed by the conductance method Phys. Med. Biol. 54, 2395-2405 (2009).
  14. Katsumoto Y, Hayashi Y, Oshige I, Omori S, Kishii N, Yasuda A and Asami K, Dielectric cytometry with three-dimensional celluar modeling Biophys. J. 95, 3043-3047 (2008)
  15. Hayashi Y, Oshige I, Katsumoto Y, Omori S, Yasuda A, Asami K, Dielectric inspection of erythrocyte morphology Phys. Med. Biol. 53, 2553-2564 (2008)
  16. Asami K, Simulation for the dielectric images of single biological cells obtained using a scanning dielectric microscope J. Phys. D: Appl. Phys. 41, 085501(1-7) (2008)
  17. Hayashi Y, Oshige I, Katsumoto Y, Omori S, Yasuda A, Asami K, Temporal variation of dielectric properties of preserved blood Phys. Med. Biol. 53, 295-304 (2008)
  18. Futaki S, Asami K, Ligand-induced Extramembrane Conformation Switch Controlling Alamethicin Assembly and the Channel Current Chemistry & Biodiversity 4, 1313-1322 (2007).
  19. Asami K., Dielectric Properties of Water in Triton X-100 (Nonionic Detergent)-Water Mixtures J. Phys. Condens. Matter 19, 376102 [1-10] (2007)
  20. Asami K, Dielectric Properties of Biological Tissues in Which Cells are Connected by Communicating Junctions J. Phys. D: Appl. Phys. 40, 3718-3727 (2007)
  21. Sekine K, Hibino C, Kimura M, Asami K, Effects of T-tubles on Dielectric Spectra of Skeletal Muscle Simulated by Boundary Element Method with Two-dimensional Models Bioelectrochemistry 70, 532-541 (2007)
  22. Asami K, Sekine K, Dielectric Modelling of Erythrocyte Aggregation in Blood J. Phys.D: Appl. Phys. 40, 2197-2204 ( 2007)
  23. Asami K, Sekine K, Dielectric Moedlling of Cell Division for Budding and Fission Yeast J. Phys. D: Appl. Phys. 40, 1128-1133 (2007)
  24. Katsumoto Y, Omori S, Yamamoto D, Yasuda A, Asami K, Dielectric Dispersion of Short Single-stranded DNA in Aqueous Solutions with and without Added Salt Phys. Rev. E 75, 011911-1-8 (2007)
  25. Okazaki T, Nagaoka Y, Asami K , Ion Channels of N-terminally-linked Alamethicin Dimers: Enhancement of Cation-Selectivity by Substitution of Glu for Gln at Position 7 Bioelectrochemistry 70, 380-386 (2007).
  26. Asami K, Effects of membrane disruption on dielectric properties of biological cells J. Phys. D: Appl. Phys. 39, 4656-4663 (2006)
  27. Kiwada T, Sonomura K, Sugiura Y, Asami K, Futaki S, Transmission of extramembrane conformational change into current: Construction of metal-gated ion channel J. Am. Chem. Soc 128, 6010-6011 (2006)
  28. Asami K, Dielectric dispersion of erythrocyte ghosts Phys. Rev. E 73, 052903-1-3 (2006)
  29. Omori S, Katsumoto Y, Asami K, Dielectric dispersion for short double-strand DNA Phys. Rev. E 73, 050901-1-4 (2006)
  30. Asami K, Dielectric dispersion in biological cells of complex geometry simulated by three-dimensional finite difference method J. Phys. D: Appl. Phys. 39, 492-499 (2006)
  31. Asami K, Simulation of dielectric relaxation in periodic binary systems of complex geometry J. Colloid Interface Sci. 292, 228-235 (2005)
  32. Asami K, Dielectric relaxation in a water-oil-Triton X-100 microemulsion near phase inversion. Langmuir 21, 9032-9037 (2005)
  33. Sekine K, Watanabe Y, Hara S, Asami K, Boundary-element calculations for dielectric behavior of doublet-shaped cells Biochim. Biophys. Acta 1721, 130-138 (2005)
  34. Okazaki T, Sakoh M, Nagaoka Y, Asami K, Ion Channels of Alamethicin Dimer N-terminally Linked by Disulfide Bond Biophys. J. 85, 267-273 (2003)
  35. Sakoh M, Okazaki T, Nagaoka Y, Asami K, N-terminal Insertion of Alamethicin in Channel Formation Studied Using Its Covalent Dimer N-terminally Linked Disulfide Bond Biochim. Biophys. Acta 1612, 117-121 (2003)
  36. Asami K, Okazaki T, Nagai Y, Nagaoka Y, Modification of alamethicin ion-channels by substitutuion of Glu7 for Gln7 Biophys. J. 83, 219-228 (2002)
  37. Sekine K, Torii N, Kuroda C, Asami K, Calculation of dielectric spectra of suspensions of rod-shaped cells using boundary element method Bioelectrochemistry 57, 83-87 (2002)
  38. Asami K, Characterization of heterogeneous systems by dielectric spectroscopy Prog. Polym. Sci. 27, 1617-1659 (2002)
  39. Asami K, Characterization of biological cells by dielectric spectroscopy J. Non-crystaline solids 305, 268-277 (2002)
  40. Asami K, Takahahsi K, Shirahige K, Progression of cell cycle monitored by dielectric spectroscopy and flow-cytometric analysis of DNA content Yeast 16, 1359-1363 (2000)
  41. Asami K, Irimajiri A, Dielectrospectroscopic monitoring of early embryogenesis in single frog embryos Phys. Med. Biol. 45, 3285 – 3297 (2000)
  42. Asami K, Effect of cell shape on dielectric behavior of fission yeast Biochim. Biophys. Acta 1472, 137-141 (1999)
  43. Asami K, Gheorghiu E, Yonezawa T, Real-time monitoring of yeast cell division by dielectric spectroscopy Biophysical J. 76, 3345-3348 (1999)
  44. Asami K, Yamaguchi T, Electrical and morphological changes of human erythrocytes under high hydrostatic pressure followed by dielectric spectroscopy Annals of Biomedical Engineering 27, 427-435 (1999)
  45. Asami K, Dielectric Relaxation Spectroscopy of Biological Cell Suspensions Handbook on Ultrasonic and Dielectric Characterization Techniques for Suspended Particulates , 333-349 (1998)
  46. Asami K, Gheorghiu E, Yonezawa T, Dielectric behavior of budding yeast in cell separation Biochim. Biophys. Acta 1381, 234-240 (1998)
  47. Asami K, Dielectric analysis of polystyrene microcapsules using scanning dielectric microscope. Colloid & Polymer Science 276, 373-378 (1998)
  48. Wada S, Iida A, Asami K, Tachikawa E, Fujita T, Role of the Gln/Glu residues of trichocellins A-II/B-II in ion-channel formation in lipid membranes and catecholamine secretion from chromaffin cells Biochim. Biophys. Acta 1325, 209-214 (1997)
  49. Koide N, Asami K, Fujita T, Ion-channels of hypelcins, antibiotic peptides, formed in planar bilayer lipid membranes Biochim. Biophys. Acta 1326, 47-53 (1997)
  50. Wada S, Iida A, Asami K, Fujita T, Ion channel-forming property of trichorovin-XII, an 11-residue peptaibol from the fungus Trichoderma viride, in planar lipid bilayer membranes Bioorganic & Medicinal Chemistry Letters 6, 2275-2278 (1996)
  51. Nagaoka Y, Iida A, Kambara T, Asami K, Tachikawa E, Fujita T, Role of proline residue in the channel-forming and catecholamine-releasing activities of the peptaibol, trichosporin-B-VIa Biochim. Biophys. Acta 1283, 31-36 (1996)
  52. Asami K, Yonezawa T, Dielectric behavior of wild-type yeast and vacuole-deficient mutant over a frequency range of 10kHz to 10GHz Biophys. J. 71, 2192-2200 (1996)
  53. Matsubara A, Asami K, Akagi A, Nishino N, Ion-channels of cyclic template-assembled alamethicins that emulate the pore structure predicted by barrel-stave model J. Chem. Soc., Chem. Commun , 2069-2070 (1996)
  54. Asami K, Yonezawa T, Wakamatsu H, Koyanagi N, Dielectric spectroscopy of biological cells Bioelectrochemistry and Bioenergetics 40, 141-145 (1996)
  55. Nagaoka Y, Iida A, Kambara T, Asami K, Fujita T, Role of Gln7 in the ion-channel-forming properties of the peptaibol trichosporin-B-VIa J. Chem. Soc., Chem. Commun , 1079-1080 (1996)
  56. Nagaoka Y, Iida A, Kambara T, Asami K, Tachikawa E, Fujita T, Ion-channel-forming and catecholamine-releasing activities of elongated and truncated analogues of trichosporin-B J. Chem. Soc., Chem. Commun. , 2203-2204 (1995)
  57. Asami K, Yonezawa T, Dielectric behavior of non-spherical cells in culture Biochim. Biophys. Acta 1245, 317-324 (1995)
  58. Asami K, Dielectric imaging of biological cells Colloid and Polymer Sci. 273, 1095-1097 (1995)
  59. Asami K, Yonezawa T, Dielectric analysis of yeast cell growth Biochim. Biophys. Acta 1245, 99-105 (1995)
  60. Nagaoka Y, Iida A, Kambara T, Tachikawa E, Asami K, Fujita T, Effect of lipophilicity of trichosporin-Bs on ion-channel formation and catecholamine-releasing activity Biol.Pharm.Bull. 18, 640-642 (1995)
  61. Asami K, Takashima S, Membrane admittance of cloned muscle cells in culture: Use of a micropipet technique Biochim. Biophys. Acta 1190, 129-136 (1994)
  62. Asami K, The scanning dielectric microscope Meas. Sci. Technol. 5, 589-592 (1994)
  63. Asami K, Zhao K S, Dielectric measurement of a single sub-millimeter size microcapsule Colloid Polym. Sci. 272, 64-71 (1994)
  64. Hanai T, Zhao K S, Asaka K, Asami K, Theoretical approach and the practice to the evaluation of structural parameters characterizing concentration polarization alongside ion-exchange membranes by means of dielectric measurements Colloid Polym. Sci. 271, 766-773 (1993)
  65. Takashima S, Asami K, Calculation and measurement of the dipole moment of small proteins: Use of protein database Biopolymers 33, 59-68 (1993)
  66. Asami K, Yamaguchi T, Dielectric spectroscopy of plant protoplasts Biophys. J. 63, 1493-1499 (1992)
  67. Zhao KS, Asaka K, Asami K, Hanai T, Dielectric analysis of concentration polarization structure at anion-exchangemembrane/solution interface under dc bias voltage application J. Colloid and Interface Science 153, 562-571 (1992)
  68. Kaneko H, Asami K, Hanai T, Dielectric analysis of sheep erythrocyte ghost. Examination of applicability of dielectric mixture equations Colloid Polym. Sci. 269, 1039-1044 (1991)
  69. Mishima K, Mimura A, Takahara Y, Asami K, Hanai T, On-line monitoring of cell concentrations by dielectric measurements J. Fermentation and Bioengineering 72, 291-295 (1991)
  70. Zhao K S, Matsubara Y, Asaka K, Asami K, Hanai T, Dielectric analysis of concentration polarization phenomena at cation-exchange membrane/solution interfaces by frequency variation and D.C. bias application J. Membrane Science 64, 163-172 (1991)
  71. Hanai T, Zhao K S, Asaka K, Asami K, Dielectric theory of concentration polarization. Relaxation of capacitance and conductance for electrolyte solution with locally varying conductivity J. Membrane Science 64, 153-161 (1991)
  72. Asami K, Takahashi Y, Takashima S, Frequency domain analysis of membrane capacitance of cultured cells HeLa and myeloma) using the micropipette technique Biophys. J. 58, 143-148 (1990)
  73. Asami K, Takahashi Y, Takashima S, Dielectric properties of mouse lymphocytes and erythrocytes Biochim. Biophys. Acta 1010, 49-55 (1989)
  74. Hanai T, Zhang H Z, Sekine K, Asaka K, Asami K, The number of interfaces and the associated dielectric relaxations in heterogeneous systems Ferroelectrics 86, 191-204 (1988)
  75. Takashima S, Asami K and Yantorno R E, Determination of electrical admittances of biological cells. J. Electrostatics 21, 225-244 (1988)
  76. Irimajiri A, Asami K, Ichinowatari T Kinoshita Y, Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. II. Effects of osmotic perturbation Biochim. Biophys. Acta 896, 214-223 (1987)
  77. Irimajiri A, Asami K, Ichinowatari T, Kinoshita Y, Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. I. Dielectric behavior of cell suspensions in 0.01-500 MHz and its simulation with a single-shell model Biochim. Biophys. Acta 896, 203-213 (1987)
  78. Asami K, Irimajiri A, Dielectric analysis of mitochondria isolated from rat liver. II. Intact mitochondria as simulated by a double-shell model Biochim. Biophys. Acta 778, 570-578 (1984)
  79. Asami K, Irimajiri A, Hanai T, Shiraishi N, Utsumi K, Dielectric analysis of mitochondria isolated from rat liver. I. Swollen mitoplasts as simulated by a single-shell model Biochim. Biophys. Acta 778, 559-569 (1984)
  80. Asami K, Irimajiri A, Dielectric dispersion of a single spherical bilayer membrane in suspension Biochim. Biophys. Acta 769, 370-376 (1984)
  81. Asami K, Hanai T, Koizumi N, Dielectric analysis of Escherichia coli suspensions in the light of the theory of interfacial polarization Biophys. J. 31, 215-228 (1980)
  82. Asami K, Hanai T, Koizumi N, Dielectric approach to suspensions of ellipsoidal particles covered with a shell in particular reference to biological cells Japanese J. Appl. Phys. 19, 359-365 (1980)
  83. Hanai T, Asami K, Koizumi N, Dielectric theory of concentrated suspension of shell-spheres in particular reference to the analysis of biological cell suspensions Bull. Inst. Chem. Res., Kyoto University 57, 297-305 (1979)

Update: Mar 22,2013