京都大学化学研究所研究者情報   [ English | Japanese ]


青山 卓史(あおやま たかし)

生体機能化学研究系 生体分子情報
(理学研究科生物科学専攻生物物理学系 協力講座)
教授
京都大学理学博士
URL:http://www.scl.kyoto-u.ac.jp/~molbio/index.html
E-mail: aoyama@scl.kyoto-u.ac.jp
Tel: 0774-38-3262
Fax: 0774-38-3259

研究・教育歴
    1985.3 京都大学大学院理学研究科博士課程退学
    1987.3 京都大学理学博士
    1985.4〜1988.3 京都大学化学研究所教務職員
    1988.4〜1994.7 京都大学化学研究所助手
    1991.7〜1993.8 米国ロックフェラー大学博士研究員
    1994.8〜2009.5 京都大学化学研究所助教授
    2009.6〜 京都大学化学研究所教授
専門分野
    分子生物学
主な研究テーマ
  1. 細胞形態形成
  2. 細胞内シグナル伝達
  3. 転写制御
主な論文・著書
  1. Tanaka, N., Uno, H., Okuda, S., Gunji, S., Ferjani, A., Aoyama, T., and Maeshima, M., SRPP, a cell wall protein is involved in development and protection of seeds and root hairds in Arabidopsis thaliana. Plant. Cell. Physiol. 58, in press (2017)
  2. Tatsumi, K., Yano, M., Kaminade, K., Sugiyama, A., Sato, M., Toyooka, K., Aoyama, T., Sato, F., and Yazaki, K., Characterization of shikonin derivative secretion in Lithospermum erythrorhizon hairy roots as a model of lipid-soluble metabolite secretion from plants. Front. Plant Sci. 7, [01066-1]-[01066-11] (2016)
  3. Wu, Z., Zhu, D., Lin, X., Miao, J., Gu, L., Deng, X., Yang, Q., Zhu, D., Cao, X., Tsuge, T., Dean, C., Aoyama, T., Gu, H., and Qu, L.-J., RNA binding proteins RZ-1B and RZ-1C play critical roles in regulating pre-mRNA splicing and gene expression during development in Arabidopsis. Plant Cell 28, 55-73 (2016)
  4. Lin, Q., Ohashi, Y., Kato, M., Tsuge, T., Gu ,H., Qu, L.-J., and Aoyama, T. , GLABRA2 directly suppresses basic helix-loop-helix transcription factor genes with diverse functions in root hair development. Plant Cell 27, 2894-2906 (2015)
  5. Wada, Y., Kusano, H., Tsuge, T., and Aoyama, T., Phosphatidylinositol phosphate 5-kinase genes respond to phosphate deficiency for root hair elongation in Arabidopsis thaliana Plant J. 81, 426-437 (2015)
  6. Hayashi, K., Nakamura, S., Fukunaga, S., Nishimura, T., Jenness, M.K., Murphy, A.S., Motose, H., Nozaki, H., Furutani, M., and Aoyama, T., Auxin transport sites are visualized in planta using fluorescent auxin analogs. Proc. Natl. Acad. Sci. USA. 111, 11557-11562 (2014)
  7. Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M., Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. J. Exp. Bot. 65, 1497-1512 (2014)
  8. Kusano, H., Tominaga, R., Wada, T., Kato, M., and Aoyama, T., Phosphoinositide signaling in root hair tip growth “Plant Cell Wall Patterning and Cell Shape” (ed., Fukuda, H.: Wiley-Blackwell) pp239-268 (2014)
  9. Zhu, D., Wu, Z., Cao, G., Li, J., Wei, J., Tsuge, T., Gu, H., Aoyama, T., and Qu, L.-J., TRANSLUCENT GREEN, an ERF family transcription factor, controls water balance in Arabidopsis by activating the expression of aquaporin genes. Mol. Plant 7, 601-615 (2014)
  10. Li, R., Li, J., Li, S., Qin, G., Novák, O., Pěnčík, A., Ljung, K., Aoyama, T., Liu, J., Murphy, A., Gu, H., Tsuge, T., and Qu, L.-J., ADP1 Affects Plant Architecture by Regulating Local Auxin Biosynthesis. PLoS Genet. 10, [e31003954-1]-[e31003954-18] (2014)
  11. Takai, A., Satoh, M., Matsuyama, T., Ito, A., Nakata, R., Aoyama, T., and Inoue, H., Secretion of miraculin through the function of a signal peptide conserved in the Kunitz-type soybean trypsin inhibitor family. FEBS Lett. 587, 1767-1772 (2013)
  12. Kato, M., Aoyama, T., and Maeshima, M., The Ca2+-binding protein PCaP2 located on the plasma membrane is Involved in root hair development as a possible signal transducer. Plant J. 74, 690-700 (2013)
  13. Lin, Q. and Aoyama, T., Pathways for epidermal cell differentiation via the homeobox gene GLABLA2: Update on the roles of the classic regulator. J. Integr. Plant Biol. 54, 729-734 (2012)
  14. Hayashi, K., Kusaka, N., Ando, K., Mitsui, T., Aoyama, T., and Nozaki, H., Design and synthesis of photolabile caged cytokinin. Bioorg. Med. Chem. Lett. 22, 5663-5667 (2012)
  15. Aki S., Nakai H., Aoyama T., Oka A., and Tsuge T., AtSap130/AtSE3b-3 function is required for reproduction in Arabidopsis thaliana. Plant Cell Physiol. 52, 1330-1339 (2011)
  16. Luo Y., Qin G., Zhang J., Liang Y., Song Y., Zhao M., Tsuge T., Aoyama T., Liu J., Gu H., and Qu L.-J., D-myo-inositol-3-phosphate affects phosphatidylinositol^mediated endomembrane function in Arabidopsis and is essential for auxin-regulated embryogenesis. Plant Cell 23, 1352-1372 (2011)
  17. She K.-C., Kusano H., Koizumi K., Yamakawa H., Hakata M., Imamura T., Fukuda M., Naito N., Tsurumaki Y., Yaeshima M., Tsuge T., Matsumoto K., Kudoh M., Itoh E., Kikuchi S., Kishimoto N., Yazaki J., Ando T., Yano M., Aoyama T., Sasaki T., Satoh H., and Shimada H., A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality. Plant Cell 22, 3280-3294 (2010)
  18. Sato K., Maki Y., Imai K.K., Aoyama T., Goto D.B., and Yamaguchi J., Control of endoreduplication of trichome by RPT2a, a subunit of the 19S proteasome in Arabidopsis. J. Plant Res. 123, 701-706 (2010)
  19. Taniguchi Y.Y., Taniguchi M., Tsuge T., Oka A., and Aoyama T., Involvement of Arabidopsis Thaliana phospholipase Dzeta2 in root hydrotropism through the supression of root gravitropism. Planta 231, 491-497 (2010)
  20. Matsuyama T., Satoh M., Nakata R., Aoyama T., and Inoue H., Functional expression of miraculin, a taste-modifying protein in Escherichia coli. J. Biochem. 145, 445-450 (2009)
  21. Aoyama T. , Phospholipid signaling in root hair development. "Root Hairs: Excellent Tools for the Study of Plant Molecular Cell Biology" (eds., Emons, A.M.C. and Ketelaar, T. Springer, Berlin Heidelberg New York) , 171-189 (2009)
  22. Dello Ioio R., Nakamura K., Moubayidin L., Perilli S., Taniguchi M., Morita M.T., Aoyama T., Costantino P., and Sabatini S., A genetic framework for the auxin/cytokinin control of cell division and diffeentiation in the root meristem. Science 322, 1380-1384 (2008)
  23. Liu J., Zhang Y., Qin G., Tsuge T., Sakaguchi N., Luo G., Sun K., Shi D., Aki S., Zheng N., Aoyama T., Oka A., Yang W., Umeda M., Xie Q., Gu H., and Qu L.-J., Targeted degradation of ICK/KRP6 by the RING type E3 ligases RHF1a and RHF2a is essential for mitotic cell cycle progression during Arabidopsis gametogenesis. Plant Cell 20, 1538-1554 (2008)
  24. Li L., Qin G., Tsuge T., Hou X., Ding M., Aoyama T., Oka A., Chen Z., Gu H., Zhao Y., and Qu L.-J., SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis. New Phytol. 179, 751-764 (2008)
  25. Kusano H., Testerink C., Vermeer J.E.M., Tsuge T., Shimada H., Oka A., Munnik T., and Aoyama T., The Arabidopsis phosphatidylinositol phosphate 5-kinase PIP5K3 is a key regulator of root hair tip growth. Plant Cell 20, 367-380 (2008)
  26. Li L., Hou X., Tsuge T., Ding M., Aoyama T., Oka A., Gu H., Zhao Y., and Qu L.-J. , The possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis. Plant Cell Rep. 27, 575-584 (2008)
  27. Taniguchi M., Sasaki N., Tsuge T., Aoyama T., and Oka A., ARR1 directly activates cytokinin response genes that encode proteins with diverse regulatory functions. Plant Cell Physiol. 48, 263-277 (2007)
  28. Imai K.K., Ohashi Y., Tsuge T., Yoshizumi T., Matsui M., Oka A., and Aoyama T., The A-type cyclin CYCA2;3 is a key regulator of ploidy levels in Arabidopsis endoreduplication. Plant Cell 18, 382-396 (2006)
  29. Ohashi Y., Oka A., Rodrigues-Pousada R., Possenti M., Roberuti I., Morelli G., and Aoyama T., Modulation of phospholipid signaling by GLABRA2 in root-hair pattern formation. Science 300, 1427-1430 (2003)
  30. Ohmiya Y., Nakai T., Park Y.W., Aoyama T., Oka A., Sakai, F., and Hayashi, T. , The role of PopCel1 and PopCel2 in poplar leaf growth and cellulose biosynthesis. Plant J. 33, 1087-1097 (2003)
  31. Aoyama T. and Oka A. , Cytokinin signal transduction in plant cells. J. Plant Res. 116, 221-231 (2003)
  32. Ohashi Y., Oka A., Ruberti I., Morelli G., and Aoyama T., Entopically additive expression of GLABRA2 alters the frequency and spacing of trichome initiation. Plant J. 29, 359-369 (2002)
  33. Ohgishi M. and Aoyama T., Inducible gene expression in plants. Molecular Plant Biology, Practical Approach (eds., Gilmartin, P.M. and Bowler, C., Oxford University Press, Oxford) 2, 97-107 (2002)
  34. Ohgishi M., Oka A., Morelli G., Ruberti I., and Aoyama T., Negative autoregulation of the Arabidopsis homeobox gene ATHB-2. Plant J. 25, 389-398 (2001)
  35. Imajuku Y., Ohashi Y., Aoyama T., Goto K., and Oka A., An upstream region of the Arabidopsis thaliana CDKA;1 (CDC2aAt) gene directs transcription during trichome development. Plant Mol. Biol. 46, 205-213 (2001)
  36. Sakai H., Honma T., Aoyama T., Sato S., Kato T., Tabata S., and Oka A., ARR1, a transcription factor for genes immediately responsive to cytokinins. Science 294, 1519-1521 (2001)
  37. Sakai H., Aoyama T., and Oka A., Arabidopsis ARR1 and ARR2 response regulators operate as transcriptional activators. Plant J. 24, 715-723 (2000)
  38. Steindler C., Matteucci A., Sessa G., Weimar T., Ohgishi M., Aoyama T., Morelli G., and Ruberti I., Shade avoidance responses are mediated by the ATHB-2 HD-Zip protein, a nagative regulator of gene expression. Development 126, 4235-4245 (1999)
  39. Aoyama T., Ohgishi M., Muramoto T., Tukuda M., and Oka A., Artificial regulation of transcription factors in transgenic plants. Proceedings of 2nd BRAIN Seminar : cytochrome P450 and plant genetic engineering , 20-23 (1999)
  40. McNellis T.W., Mudgett M.B., Li K., Aoyama T., Horvath D., Chua N.-H., and Staskawicz B.J., Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic arabidopsis induces hypersensitive cell death. Plant J. 14, 247-257 (1998)
  41. Sakai H., Aoyama T., Bono H., and Oka A., Two-component response regulators from Arabidopsis thaliana contain a putative DNA-binding motif. Plant Cell Physiol. 39, 1232-1239 (1998)
  42. Liang Y., Aoyama T., and Oka A., Structural characterization of the virB operon on the hairy-root-inducing plasmid A4. DNA Res. 5, 87-93 (1998)
  43. Aoyama T., Glucocorticoid-inducible gene expression in plants. Inducible Gene Expression in Plants (ed., Reynolds, P., CAB International, Wallingford) , 44-59 (1998)
  44. Aoyama T. and Chua N.-H., A Gulucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J. 11, 605-612 (1997)
  45. Nevalainen L.T., Aoyama T., Ikura M., Crivici A., Yan H., Chua N.-H., and Nairn A.C., Characterization of novel calmodulin-binding peptides with distinct inhibitory effects on calmodulin-dependent enzymes. Biochem J. 321, 107-115 (1997)
  46. Aoyama T., Dong C.-H., Wu Y., Carabelli M., Sessa G., Ruberti I., Morelli G., and Chua N.-H., Ectopic expression of the Arabidopsis transcription activator Athb-1 alters leaf cell fate in tobacco. Plant Cell 7, 1773-1785 (1995)
  47. Morelli G., Aoyama T., Dong C.-H., Lucchetti S., Carabelli M., Sessa G., Ruberti I., and Chua N.-H., The expression and function of the Arabidopsis ATHB-1 gene during plant development. J Cell Biochem. 21A, 458-458 (1995)
  48. Oka A., Aoyama T., and Endoh H., Review: Transcriptional control of the Agrobacterium virulence genes by two proteins VirA and VirG. Bull. Institute Chemical Research Kyoto University 71, 355-367 (1993)
  49. Aoyama T., Endoh H., Takanami M., and Oka A., DNA signal architecture for transcriptional activation by the regulatory protein VirG. Bull. Inst. Chem. Res. Kyoto Univ. 71, 288-294 (1993)
  50. Endoh H., Aoyama T., and Oka A., Transcription in vitro promoted by the Agrobacterium VirG protein. FEBS Let. 334, 277-280 (1993)
  51. Ichikawa A., Kuboya T., Aoyama T., and Sugiura Y., Activation of DNA cleavage by dynemicin A in a B-Z conformational junction. Biochemistry 31, 6784-6787 (1992)
  52. Gash A., Aoyama T., Foster R., and Chua N.-H., Gene isolation with the polymerase chain reaction. Methods in Arabidopsis Research (eds., Koncz, C., Chua, N.-H., and Schell, J., World Scientific Publishing Co., New Jersey) , 342-356 (1992)
  53. Aoyama T., Takanami M., Makino K., and Oka A., Cross-talk between the virulence and phosphate regulons of Agrobacterium tumefaciens caused by unusual interaction of the transcriptional activator with a regulatory DNA element. Mol. Gen. Genet. 227, 385-390 (1991)
  54. Tamamoto S., Aoyama T., Takanami M., and Oka A. , Binding of the regulatory protein VirG to the phased signal sequences upstream from virulence genes on the hairy-root inducing plasmid. J. Mol. Biol. 215, 537-547 (1990)
  55. Endoh H., Hirayama T., Aoyama T., and Oka A., Characterization of the virA gene of the agropine-type plasmid pRiA4 of Agrobacterium rhizogenes. FEBS Let. 271, 28-32 (1990)
  56. Aoyama T. and Oka A., Review hypothesis: A common mechanism of transcriptional activation by the three positive regulators, VirG, PhoB, and OmpR. FEBS Let. 263, 1-4 (1990)
  57. Aoyama T., Hirayama T., Tamamoto S., and Oka A., Putative start codon TTG for the regulatory protein VirG of the hairy-root-inducing plasmid pRiA4. Gene 78, 173-178 (1989)
  58. Aoyama T., Takanami M., and Oka A., Signal structure for transcriptional activation in the upstream regions of virulence genes on the hairy-root-inducing plasmid A4. Nucleic Acids Res. 21,8711-8725 (1989)
  59. Aoyama T., Takanami M., and Oka A., Signal structure of the cis-acting element recognized by VirG protein, a positive regulator in Agrobacterium. Nucleic Acids Symp. Series. 21, 17-18 (1989)
  60. Aoyama T. and Takanami M., Supercoiling response of E.coli promoters with different spacer lengths. Biochim. Biophys. Acta 949, 313-317 (1988)
  61. Nagahara K., Miyake Y., Aoyama T., and Ogino F., Tissue oxygen tension in the stria vascularis. Acta Otolaryngol. 456, 137-142 (1988)
  62. Nagahara K., Aoyama T., Fukuse S., and Noi O., Effects of prostaglandins on perilymphatic oxygenation. Acta Otolaryngol. 456, 143-150 (1988)
  63. Aoyama T., Review: Essential structure of E.coli promoter. Bull. Inst. Chem. Res. Kyoto Univ. 64, 320-338 (1986)
  64. Nagahara K., Miyake Y., Naito Y., Yoza T., Fukushima H., and Aoyama T., Evaluation of statokinesigram using 3-D plotting and discriminant. Acta Otolaryngol. 419, 79-85 (1985)
  65. Aoyama T. and Takanami M., Essential structure of E. coli promoter: Effect of sequences around the RNA start point on promoter function. Nucleic Acids Res. 13, 4085-4096 (1985)
  66. Aoyama T., Takanami M., Ohtsuka E., Taniyama Y., Marumoto R., Sato H., and Ikehara M., Essential structure of E. coli promoter: Effect of spacer length between the two consensus sequences on promoter function. Nucleic Acids Res. 11, 5855-5864 (1983)


Update: Jul 18,2017