Faculty Information, Institute for Chemical Research, Kyoto University   [ English | Japanese ]


NAKASE, Ikuhiko

Division of Biochemistry - Biofunctional Design-Chemistry -
（Laboratory in the Kyoto University）
Assistant Professor
PhD
URL:
E-mail: nakase@scl.kyoto-u.ac.jp
Tel: 0774-38-3211
Fax: 0774-32-3038
Academic career
    2005.3 PhD, Graduate School of Pharmaceutical Sciences, Kyoto University
    2005.4-2006.3 Postdoctoral Researcher, Department of Chemistry, University of Washington
    2006.4- Assistant Professor, Institute for Chemical Research, Kyoto University
Research field
    Peptide and protein chemistry, cellular engineering
Current research
  1. Arginine-rich peptides: mechanisms of intracellular delivery and its applications
  2. Design and synthesis of biofunctional peptides
Selected publications
  1. Nakase, I.; Okumura, S.; Tanaka, G.; Osaki, K.; Imanishi, M.; Futaki, S., Signal Transduction of Biological Systems through an Artificial Receptor System via Dimerization of a Bivalent Leucine-Zipper Ligand Angew. Chem. Int. Ed. 51, 7464-7467 (2012)
  2. Nakase, I.; Okumura, S.; Katayama, S.; Hirose, H.; Pujals, S.; Yamaguchi, H.; Arakawa, S.; Shimizu, S.; Futaki, S., Transformation of an Antimicrobial Peptide into a Plasma Membrane-Permeable, Mitochondria-Targeted Peptide via the Substitution of Lysine with Arginine Chem. Commun. 48, 11097-11099 (2012)
  3. Tanaka, G.; Nakase, I.; Fukuda, Y.; Masuda, R.; Oishi, S.; Shimura, K.; Kawaguchi, Y.; Takatani-Nakase, T.; Langel, Ü.; Gräslund, A.; Okawa, K.; Matsuoka, M.; Fujii, N.; Hatanaka, Y.; Futaki, S., CXCR4 Stimulates Macropinocytosis: Implications for Cellular Uptake of Arginine-Rich Cell-Penetrating Peptides and HIV Chem. Biol. (in press)
  4. Nakase, I.; Konishi, Y.; Ueda, M.; Saji, H.; Futaki, S., Accumulation of Arginine-Rich Cell-Penetrating Peptides in Tumors and the Potential for Anticancer Drug Delivery in Vivo J. Control. Release 159, 181-188 (2012)
  5. Nakase, I.; Akita, H.; Kogure, K.; Gräslund, A.; Langel, U.; Harashima, H.; Futaki, S., Efficient Intracellular Delivery of Nucleic Acid Pharmaceuticals Using Cell-Penetrating Peptides Acc. Chem. Res. 45, 1132-1139 (2012)
  6. Futaki, S.; Hirose, H.; Nakase, I., Arginine-Rich Peptides: Methods of Translocation through Biological Membranes Curr. Pharm. Des. (in press)
  7. Kuroda, Y.; Kato-Kogoe, N.; Tasaki, E.; Murata, E.; Ueda, K.; Abe, M.; Miyamoto, K.; Nakase, I.; Futaki, S.; Tohyama, Y.; Hirose, M., Oligopeptides Derived from Outiphosphorylation Sites of EGF Receptor Suppress EGF-Stimulated Responses in Human Lung Carcinoma A549 Cells Eur. J. Pharmacol. (in press)
  8. Sakamoto, K.; Aburai, K.; Morishita, T.; Sakai, K.; Sakai, H.; Abe, M.; Nakase, I.; Futaki, S., Bio-Inspired Mechanism for the Translocation of Peptide through the Cell-Membrane Chem. Lett. (in press)
  9. Takayama, K.; Hirose, H.; Tanaka, G.; Pujals, S.; Katayama, S.; Nakase, I.; Futaki, S., The Effect of the Attachment of a Penetration Accelerating Sequence and the Influence of Hydrophobicity on Octaarginine-Mediated Intracellular Delivery Mol. Pharm. 9, 1222-1230 (2012)
  10. 中瀬生彦、田中弦、二木史朗, 膜透過性アルギニンペプチドの設計と合成 遺伝子医学 MOOK「最新ペプチド合成技術とその創薬研究への応用」 173-178 (2012)
  11. Hirose, H.; Takeuchi, T.; Osakada, H.; Pujals, S.; Nakase, I.; Kobayashi, S.; Haraguchi, T.; Futaki, S., Transient Focal Membrane Deformation Induced by Arginine-rich Peptides Leads to Their Direct Penetration into Cells Mol. Ther. 20, 984-993 (2012)
  12. 二木史朗、中瀬生彦, ペプチドベクターを用いた効率的細胞内導入法（第19章） CMC出版「蛍光イメージング／MRIプローブの開発」（分担）
  13. 二木史朗、中瀬生彦, オリゴアルギニン修飾を用いた薬物の細胞内取り込みの改善とその機構 遺伝子医学MOOK 別冊「ペプチド・タンパク質性医薬品の新規DDS製剤の開発と応用」（分担）
  14. Nakase I, Kogure K, Harashima H, Futaki S, Application of a Fusiogenic Peptide GALA for Intracellular Delivery Methods Mol Biol 683, 525-533 (2011)
  15. Katayama S, Hirose H, Takayama K, Nakase I, Futaki S, Acylation of Octaarginine: Implication to the Use of Intracellular Delivery Vectors J Control Release 149, 29-35 (2011)
  16. Miyamoto R, Akizawa H, Nishikawa T, Uehara T, Azuma Y, Nakase I, Futaki S, Hanaoka H, Iida Y, Endo K, Arano Y, Enhanced Target-Specific Accumulation of Radiolabeled Antibodies by Conjugating Arginine-Rich Peptides as Anchoring Molecules Bioconjug Chem 21, 2031-2037 (2010)
  17. Yukawa H, Noguchi H, Nakase I, Miyamoto Y, Oishi K, Hamajima N, Futaki S, Hayashi S, Transduction of Cell-penetrating Peptides into Induced Pluripotent Stem Cells Cell Transplant 19, 901-909 (2010)
  18. Shimane K, Kodama EN, Nakase I, Futaki S, Sakurai Y, Sakagami Y, Li X, Hattori T, Sarafianos SG, Matsuoka M, Rev-derived Peptides Inhibit HIV-1 Replication by Antagonism of Rev and a Co-receptor, CXCR4. Int J Biochem Cell Biol 42, 1482-1488 (2010)
  19. Nakase I, Kobayashi S, Futaki S, Endosome-disruptive Peptides for Improving Cytosolic Delivery of Bioactive Macromolecules Biopolymers 94, 763-770 (2010)
  20. Yu HH, Nakase I, Pujals S, Hirose H, Tanaka G, Katayama S, Imanishi M, Futaki S, Expressed Protein Ligation for the Preparation of Fusion Proteins with Cell Penetrating Peptides for Endotoxin Removal and Intracellular Delivery BBA-Biomembranes 1798, 2249-2257 (2010)
  21. 二木史朗, 中瀬生彦, 膜透過性塩基性ペプチドを用いる細胞内送達技術～その分子機構と応用～ 生物物理 50, 137-140 (2010)
  22. Nakase I, Hirose H, Tanaka G, Tadokoro A, Kobayashi S, Takeuchi T, and Futaki S, Cell Surface Accumulation of Flock House Virus-Derived Peptide Leads to Efficient Internalization via Macropinocytosis Mol. Ther. 17, 1868-1876 (2009).
  23. Lai H, Nakase I, Lacoste E, Singh NP, Sasaki T, Artemisinin-Transferrin Conjugate Retards Growth of Breast Tumors in the Rat Anticancer Res. 29, 3807-3810 (2009).
  24. Gao C, Izquierdo-Barba I, Nakase I, Futaki S, Ruan J, Sakamoto K, Sakamoto Y, Kuroda K, Terasaki O, Che S, Mesostructured Silica Based Delivery System for a Drug with a Peptide as a Cell-Penetrate Vector Micropor. Mesopor. Mat. 122, 201-207 (2009).
  25. Takayama K, Nakase I, Michiue H, Takeuchi T, Tomizawa K, Matsui H, Futaki S, Enhanced Intracellular Delivery Using Arginine-Rich Peptides by the Addition of Penetration Accelerating Sequences (Pas) J Control Release 138, 128-133 (2009).
  26. Watkins LC, Brennan P, Fegan C, Takayama K, Nakase I, Futaki S, Jones AT, Cellular Uptake, Distribution and Cytotoxicity of the Hydrophobic Cell Penetrating Peptide Sequence PFVYLI Linked to the Proapoptotic Domain Peptide PAD J Control Release 140, 237-244 (2009).
  27. Kobayashi S, Nakase I, Kawabata N, Yu H, Pujals S, Imanishi M, Giralt E, Futaki S, Cytosolic Targeting of Macromolecules Using a pH-Dependent Fusogenic Peptide in Combination with Cationic Liposomes Bioconj Chem 20, 953-959 (2009).
  28. Inomata K, Ohno A, Tochio H, Isogai S, Tenno T, Nakase I, Takeuchi T, Futaki S, Ito Y, Hiroaki H, Shirakawa M, Multi-Dimensional NMR Spectroscopy of Proteins in Living Human Cells Nature 458, 106-109 (2009)
  29. Takayama K, Tadokoro A, Pujals S, Nakase I, Giralt E, Futaki S, Novel System to Achieve One-Pot Modification of Cargo Molecules with Oligoarginine Vectors for Intracellular Delivery Bioconj Chem 20, 249-257 (2009)
  30. Nakase I, Gallis B, Takatani-Nakase T, Oh S, Lacoste E, Singh NP, Goodlett DR, Tanaka S, Futaki S, Lai H, Sasaki T, Transferrin Receptor-Dependent Cytotoxicity of Artemisinin-Transferrin Conjugates on Prostate Cancer Cells and Induction of Apoptosis Cancer Lett. 274, 290-298 (2009).
  31. Kosuge M, Takeuchi T, Nakase I, Jones A T, Futaki S, Cellular Internalization and Distribution of Arginine-Rich Peptides as a Function of Extracellular Peptide Concentration, Serum, and Plasma Membrane Associated Proteoglycans Bioconjug. Chem. 19, 656-664 (2008).
  32. Nakase I, Takeuchi T, Tanaka G, Futaki S, Methodological and Cellular Aspects That Govern the Internalization Mechanisms of Arginine-Rich Cell-Penetrating Peptides Adv. Drug Deliv. Rev. 60, 598-607 (2008).
  33. Nakase I, Lai H, Singh N P, Sasaki T, Anticancer Properties of Artemisinin Derivatives and Their Targeted Delivery by Transferrin Conjugation Int. J. Pharm. 354, 28-33 (2008).
  34. Futaki S, Nakase I, Tadokoro A, Takeuchi T, Jones A T, Arginine-rich Peptides and Their Internalization Mechanisms Biochem. Soc. Trans. 35, 784-787 (2007).
  35. Kameyama S, Horie M, Kikuchi K, Omura T, Tadokoro A, Takeuchi T, Nakase I, Sugiura Y, Futaki S, The Use of Acid Wash in Determining Cellular Uptake of Fab/Cell-Permeating Peptide Conjugates Biopolymers 88, 98-107 (2007).
  36. Fretz M M, Penning N A, Al-Taei S, Futaki S, Takeuchi T, Nakase I, Storm G, Jones A T, Temperature, Concentration and Cholesterol Dependent Translocation of L- and D-Octaarginine Across the Plasma and Nuclear Membrane of CD34 + Leukaemia Cells Biochem. J. 403, 335-342 (2007).
  37. Nakase I, Tadokoro A, Kawabata N, Takeuchi T, Katoh H, Hiramoto K, Negishi M, Nomizu M, Sugiura Y, Futaki S, Interaction of Arginine-Rich Peptides with Membrane-Associated Proteoglycans Is Crucial for Induction of Actin Organization and Macropinocytosis Biochemistry 46, 492-501 (2007).
  38. Fretz M, Jin J, Conibere R, Penning N A, Al-Taei S, Storm G, Futaki S, Takeuchi T, Nakase I, Jones A T, Effects of Na(+)/H(+) Exchanger Inhibitors on Subcellular Localisation of Endocytic Organelles and Intracellular Dynamics of Protein Transduction Domains HIV-TAT Peptide and Octaarginine J. Control. Release 116, 247-254 (2006).
  39. Kameyama S, Horie M, Kikuchi T, Omura T, Takeuchi T, Nakase I, Sugiura Y, Futaki S, Effects of Cell-permeating Peptide Binding on the Distribution of 125I-labeled Fab Fragment in Rats Bioconjug. Chem. 17, 597-602 (2006).
  40. Al-Taei S, Penning N A, Simpson J C, Futaki S, Takeuchi T, Nakase I, Jones A T, Intracellular Traffic and Fate of Protein Transduction Domains HIV-1 TAT Peptide and Octaarginine. Implications for Their Utilization as Drug Delivery Vectors Bioconjug. Chem. 17, 90-100 (2006).
  41. Kameyama S, Okada R, Kikuchi T, Omura T, Nakase I, Takeuchi T, Sugiura Y, Futaki S, Distribution of Immunoglobulin Fab Fragment Conjugated with HIV-1 REV Peptide Following Intravenous Administration in Rats Mol. Pharm. 3, 174-180 (2006).
  42. Futaki S, Masui Y, Nakase I, Sugiura Y, Nakamura T, Kogure K, Harashima H, Unique Features of a pH-sensitive Fusogenic Peptide that Improves the Transfection Efficiency of Cationic Liposomes J. Gene Med. 7, 1450-1458 (2005).
  43. Futaki S, Nakase I, Suzuki T, Nameki D, Kodama E, Matsuoka M, Sugiura Y, RNase S Complex Bearing Arginine-rich Peptide and Anti-HIV Activity J. Mol. Recognit. 18, 169-174 (2005).
  44. Nakase I, Niwa M, Takeuchi T, Sonomura K, Kawabata N, Koike Y, Takehashi M, Tanaka S, Ueda K, Simpson J C, Jones A T, Sugiura Y, Futaki S, Cellular Uptake of Arginine-rich Peptides: Roles for Macropinocytosis and Actin Rearrangement Mol. Ther. 10, 1011-1022 (2004).
  45. Kakudo T, Chaki S, Futaki S, Nakase I, Akaji K, Kawakami T, Maruyama K, Kamiya H, Harashima H, Transferrin-modified Liposomes Equipped with a pH-sensitive Fusogenic Peptide: An Artificial Viral-like Delivery System Biochemistry 43, 5618-5628 (2004).
  46. Futaki S, Niwa M, Nakase I, Tadokoro A, Zhang Y, Nagaoka M, Wakako N, Sugiura Y, Arginine Carrier Peptide Bearing Ni(II) Chelator to Promote Cellular Uptake of Histidine-tagged Proteins Bioconjug. Chem. 15, 475-481 (2004).
  47. Futaki S, Zhang Y, Kiwada T, Nakase I, Yagami T, Oki S, Sugiura Y, Gramicidin-based Channel Systems for the Detection of Protein-ligand Interaction Bioorg. Med. Chem. 12, 1343-1350 (2004).
  48. Futaki S, Goto S, Suzuki T, Nakase I, Sugiura Y, Structural Variety of Membrane Permeable Peptides Curr. Protein Pept. Sci. 4, 87-96 (2003).
  49. Futaki S, Nakase I, Suzuki T, Youjun Z, Sugiura Y, Translocation of Branched-Chain Arginine Peptides through Cell Membranes: Flexibility in the Spatial Disposition of Positive Charges in Membrane-Permeable Peptides Biochemistry 41, 7925-7930 (2002).
  50. Futaki S, Araki M, Kiwada T, Nakase I, Sugiura Y, A Cassette RNase: Site-Selective Cleavage of RNA by RNase S Equipped with RNA-Recognition Segment Bioorg. Med. Chem. Lett. 11, 1165-1168 (2001).

Update: Nov 14,2012